Microbiological study of eikenella corrodens isolated from patients and healthy individuals

A total of 99 strains of E. corrodens were isolated in this research. Seventy strains were isolated as flora from 392 normal individuals [17.85%] and 29 strains from 539 in pure form in 2 of the 29 clinical specimens [6.8%], otherwise It was mixed with other bacteria. Most of the isolations were from the mouth [74] but the organism was also recovered from 11 wounds, 7 abscesses and 7 samples of sputum, fluid and transtracheal aspirate. The most common form of E. corrodens infection was the clenched first injuries [33.3%] followed by human bite wounds [20%] and periodontitis [20%]. It is found that colonies of only 71.7% of our isolates were pitting the surface. A comparative growth was studied on 10 media incubated in 3 atmospheres. Chocolate and blood agar found to be the best media using 5% CO[2], Fifty strains tested for 9 biochemical reactions, gave typical results as found by other authors. MIC has been done for 20 strains by the agar dilation and microtiter broth dilution methos E. corrodens found highly susceptible to penicillin and cefoxitin but fairly resistant to clindamycin

Experimental association of infection by salmonella and schistosoma mansoni in Swiss Albino mice

Sixty swiss males albino mice from the same breed, were used in this study. They were classified into four groups [I] normal non-infected control group [10 mice][II] control group infected by Salmonella typimurium organism [10 mice][III] control group infested by Sch. mansoni [20 mice] and [IV] a study group infected by both Sch. mansoni and Salmonella typimurium [20 mice] The relation between schistosomiasis and salmonella organism was evaluated by egg-count per gram stool and per gram liver tissue and by studying the worm load by worm perfusion method. The relation was also evaluated by a histopathological study for the liver, spleen and intestine and by a bacteriological study including culture of the perfused worms, liver and spleen. The study demonstrated: 0.99% reduction of egg-count per gram stool and 0.61% reduction of egg-count per gram liver tissue in the group [IV] compared to the third one. Histo pathological study revealed reduction of bilharzial granulomas in the liver of group [IV] compared to the third group. Regarding the bacteriological study, the most important result was the presence of salmonella growth in 60% of cultures of perfused worms from group [IV]

prevalence of vaginal candidiasis with different methods of contraception

This study included 110 women classified into 3 groups; 30 using oral pills, 40 using intrauterine device [lUD] and 40 using no method of contraception as a control group. High vaginal swabs were taken; smears prepared, stained with gram and cultures made on Sabouraud Dextrose agar with chloramphenicol. Isolated yeast colonies were identified by germ tube formation test, API-20 c system and morphology on cornmeal tween 80 agar. Candida species have been isolated in 22. 5% of IUD users group, 20% in pins users group and 15% in the control group. Candida albicans could be isolated in 68.2% out of 22 isolates from all cases, C. glabrata in 27.3% and C. krusei in 4.5%. Candida albicans found to be the most prevalent species in the studied groups of women

Incidence of Oral Candida in Children under corticosteroid therapy and the use of chlorhexidine mouth wash as a preventive measure

In this study 60 children indicated for corticosteroid therapy were classified according to the mouth wash into: Group [I]: Chlorhexidine 0.2% mouth wash [30 cases] Group [II]: Saline mouth wash [30 cases]. Further subdivision was done according to mode of corticosteroid administration; 20 cases in each group under systemic therapy while 10 cases in each group under inhaled therapy. Cases were examined clinically for signs and symptoms of oral candidiasis. Microbiological examination included mucosal smear, culture, viable candidal count and identification of Candida albicans by germ tube and morphology on cornmeal tween 80.There is one case under systemic steroid therapy and using chlorhexidine mouth wash that developed clinical signs and symptoms of candidiasis compared to two cases under the same therapy but using saline as a mouth wash. No manifestations found in cases under inhaled steroid therapy either using chlorhexidine or saline. There was no increase of positive culture in patients under inhaled therapy using chlorhexidine or chlorhexidine or saline. While in patients under systemic steroid it increased to reach 65% with chlorhexidine and 85% with saline mouth wash. Incidence of Candida albicans infection was high [ranged from 75-100% of positive cultures] compared with other Candida species [0-25%]. There is an increase in the incidence of carrier and diseased patients according to the viable candidal count ranging from 10% to 45%