ABSTRACT
Aim: Evaluation of biological characteristics of 13 identified proteins of patients with cirrhotic liver disease is the main aim of this research
Background: In clinical usage, liver biopsy remains the gold standard for diagnosis of hepatic fibrosis. Evaluation and confirmation of liver fibrosis stages and severity of chronic diseases require a precise and noninvasive biomarkers. Since the early detection of cirrhosis is a clinical problem, achieving a sensitive, specific and predictive novel method based on biomarkers is an important task
Methods: Essential analysis, such as gene ontology [GO] enrichment and protein-protein interactions [PPI] was undergone EXPASy, STRING Database and DAVID Bioinformatics Resources query
Results: Based on GO analysis, most of proteins are located in the endoplasmic reticulum lumen, intracellular organelle lumen, membrane-enclosed lumen, and extracellular region. The relevant molecular functions are actin binding, metal ion binding, cation binding and ion binding. Cell adhesion, biological adhesion, cellular amino acid derivative, metabolic process and homeostatic process are the related processes. Protein-protein interaction network analysis introduced five proteins [fibroblast growth factor receptor 4, tropomyosin 4, tropomyosin 2 [beta], lectin, Lectin galactoside-binding soluble 3 binding protein and apolipoprotein A-I] as hub and bottleneck proteins
Conclusion: Our result indicates that regulation of lipid metabolism and cell survival are important biological processes involved in cirrhosis disease. More investigation of above mentioned proteins will provide a better understanding of cirrhosis disease
ABSTRACT
Angiotensin I converting enzyme [ACE] is a Zinc metalloproteinase, converts Ang I to Ang- IIa pro-inflammatory agent which may contribute to pathophysiology of some diseases like type 2 diabetes. To investigate the relationship between ACE I/D polymorphism and type 2 diabetes in 261 Iranian casecontrol pairs. 170 patients [85 type 2 diabetics with nephropathy and 85 type 2 diabetics without nephropathy] and 91 healthy control subjects were enrolled in our study. I/D polymorphism of the ACE gene was detected by polymerase chain reaction [PCR] utilizing specific primers. The frequency of DD genotype in the DN group was higher than that of the type 2 diabetic patients [30.6% vs. 20%, P = 0.157] and the control group [30.6% vs. 14.3%, P=0.006]. The frequency of D allele in nephropathic patients was 58.2% as compared to type 2 diabetic patients without nephropathy 50.5% [P=0.19] and control subjects 37.3% [P =0.001]. Therefore, the frequency of DD genotype and D allele significantly increased in DN patients in comparison to healthy controls. It is concluded that the DD genotype and /or D allele of ACE gene may increase the risk for type 2 diabetes but not diabetic nephropathy