ABSTRACT
Background and Objective: A brief and short duration episode of ischemia is recorded in ischemic preconditioning [IPC]. This latter condition provides a status in which large region of heart is protected when prolonged ischemia occurred. Levothyroxine play a protective role in IPC induction, and simultaneously with stress oxidative. This study was conducted to determine the protective effect of levothyroxine with oxidative stress reduction mechanism in ischemic preconditioning model in rat heart
Methods: This experimental study was performed on 30 male Wistar rats in three groups of 10, as follows. In the reperfusion ischaemia group [IR], the heart of the animal was placed in a Langendorff apparatus. In the ischemic preconditioning group [IPC], prior to major ischemia, was exposed to 4 periods of 5-minute ischemia with reperfusion. In the intraperitoneally administered group, levothyroxine at a dose of 25 microgram per 100 g of body weight, the heart was exposed to reperfusion ischemia. The area of infarct and the level of malondialdehyde in the heart tissue were measured
Results: The volume of Infarcted region in IR and IPC groups was 26.55 and 11.11 respectively. The same index for the Levothyroxine receiver was 12.56. Based on these findings it was demonstrated that Levothyroxine injection reduced the Infarcted region significantly similar with IPC [P<0.05]. The MDA Levels in IR and IPC were 1328 and 777, respectively and in Levothyroxine group it was determined as 762. The size of Infarcted region in both IPC and treated with Levothyroxine groups significantly reduced in compared to IR group [P<0.05]
Conclusion: Injection of levothyroxine with ischemic preconditioning reduced the effect of reperfusion maladaptive ischemia in rat heart
ABSTRACT
Objective: there are limited studies on the relationship between inflammatory marker such as IL-6 and lipid peroxidation and metabolic syndrome. The aim of present study was to assess IL-6 and lipid peroxidation in subjects with and without the metabolic syndrome and their association with metabolic syndrome components
Methodology: age and gender matched 40 subjects with metabolic syndrome and 40 control groups took part in this study
Results: the mean malondialdehyde level was significantly higher in over-weight and obese subjects with metabolic syndrome than control groups [P<0.05]. The mean level of IL-6 in men and the mean level of malondialdehyde in women with metabolic syndrome was significantly higher than control groups [p < 0.05]. There were significant positive correlation between malondialdehyde and fasting blood glucose, triglyceride and systolic blood pressure [p<0.05]
Conclusion: our results suggest that higher levels of IL-6 and malondialdehyde may cause insulin resistance and metabolic disorders in all subjects with metabolic syndrome. Malondialdehyde level shows strong association with some metabolic syndrome components. This means the greater risk of metabolic syndrome
ABSTRACT
Different findings indicate that CYP2C plays a clinical role in determining interindividual and interethnic differences in drug effectiveness. The ethnic differences in the frequency of CYP2C19 mutant alleles continue to be a significant study topic. The aim of the present study was to assess the frequency of allelic variants of CYP2C19 in Turkman ethnic groups and compare them with the frequencies in other ethnic populations. The study group included 140 unrelated healthy ethnic Turkman subject referred to the Health Center. Genotyping of CYP2C19 alleles [CYP2C19*1, CYP2C19*2, and CYP2C19*3 alleles] was carried out by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism technique. The allele frequency of CYP2C19*1, CYP2C19*2 and CYP2C19*3 were 56.43%, 23.57% and 20%, respectively. The result also showed that 39.7% of subjects expressed the CYP2C19*1/*1 genotype. While 42.1%, 9.3%, 9.3% and 1.4% expressed CYP2C19*1/*2, CYP2C19*1/*3, CYP2C19*2/*2 and CYP2C19*3/*3 genotypes, respectively. The genotype CYP2C19*2/*3 was not expressed in this study population. The findings suggested that 10% of subjects were poor metabolizers by expressing CYP2C19*2/*2 and CYP2C19*3/*3 genotypes. Fifty one percent of subjects were intermediate metabolizers having CYP2C19*1/*2, CYP2C19*2/*3 and CYP2C19*1/*3 genotypes and 37.86% were found to be extensive metabolizers expressing CYP2C19*1/*1 genotype. The frequency of intermediate metabolizers genotype was high [51%] in Turkman ethnic groups. This study showed that the determined allelic variants of CYP2C19 [CYP2C19*2 and CYP2C19*3 mutations] in Turkman ethnic group are comparable to other populations. These findings could be useful for the clinicians in different country to determine optimal dosage and effectiveness of drugs metabolized by this polymorphic enzyme
Subject(s)
Humans , Female , Male , Ethnicity , Polymerase Chain Reaction , Polymorphism, GeneticABSTRACT
This study was undertaken to determine the effect of subacute exposure of peracetic acid on lipid peroxidation and hepatic enzymes in Wistar rats. 48 male animals in Treatment Group I, II and III received 0.2%, 2% and 20% peracetic acid daily for 2 and 4 weeks. Serum malondialdehyde increased and Alanine Transaminase and Aspartate Transaminase decreased significantly in groups 2 and 3, compared to the control group. The malondialdehyde, Alanine Transaminase and Aspartate Transaminase with 0.2% and 2% doses of peracetic acid for 2 weeks do not lead to the alteration of malondialdehyde and enzyme activities. This study demonstrated that the enhancement of malondialdehyde could provide an oxidative damage induced by disinfectant peroxidation at 20% and 2% doses at 2 and 4 weeks. The consumption of peroxidation with 20% for 2 weeks and 2% for 4 weeks can cause the increase of malondialdehyde and the decrease of enzyme activities, respectively
ABSTRACT
The incidence of thyroid cancer is increasing in several countries. The main aim of this study was to find and describe province-specific estimates of incidence in males and females by age groups for thyroid cancer. The data used in this study were collected from a cancer registry that was established by Health Deputy of Golestan province for a period of one year [2004], in different age groups. Thyroid cancer data was identified and collected through the eighteen Pathology Laboratory centers [where males and female populations is referred] in Golestan province. A total of 348 females and 409 males cases with cancer representing all sites were identified during the study period. It included seventeen females and five male thyroid cancer patients. In female's thyroid cancer, there were twelve papillary carcinoma [70.6%], two medullary carcinoma, one carcinoma anaplastic and one carcinoma [5.9%]. In male's, there were two papillary carcinoma [40%] and one follicular carcinoma. The incidence of thyroid cancer in 70-79 age groups for females and males was the highest and lowest in age group 10-19 years' in females and 30-39 years in males. The incidence of thyroid cancer is higher in females when compared with males as per this one year study. However since this is just one year data, it needs to be studied further to confirm these findings
Subject(s)
Humans , Male , Female , Incidence , Carcinoma, Papillary , Carcinoma, MedullaryABSTRACT
To evaluate concentration-dependent effects of total extract of Ruta graveolens and its purified alkaloid fraction on the nodal basic and functional properties. In the present experimental study, we used the Langendorff model for perfusion of isolated rat hearts to determine the effects of various concentrations of methanolic extract of Rue 1.25x10-6% weight per volume percent [W/V]; 2.5x10-6% W/V; 3.75x10-6% W/V and total alkaloid of Rue 0.25x10-6% W/V; 0.5x10-6% W/V on electrophysiological properties of cardiac tissue. Selective stimulation protocols were used to independently quantify atrioventricular AV nodal recovery, facilitation, and fatigue. We used 3 groups N=24 of isolated perfused rat AV nodal preparations to assess the effect of Rue extracts. The study was carried out in October 2006 in the electrophysiology laboratory of the Cardiovascular Research Center of Golestan University of Medical Sciences, Golestan, Gorgan, Iran. Our results showed that both the total plant extract and the alkaloid fraction of Ruta graveolens had a similar trend of action on nodal conduction time and refractoriness. Furthermore, we observed increased atrioventricular conduction time 83 +/- 4 to 108 +/- 5 msec and functional refractory period 157.6 +/- 3 to 163.7 +/- 4 msec at a maximum concentration of 3.75x10-6% W/V. The above results indicated a potential antiarrhythmic effect of Ruta graveolens in treating supra ventricular tachyarrhythmia