ABSTRACT
The dried plant was extracted with dichloromethane and after defatting with hexane, transferred repeatedly on silica columns using dichloromethane-hexane and ethyl acetate-hexane as mobile phases. Finally the fractions were purified by high performance liquid chromatography using a Pack-Sil column and hexane: Ethyl acetate as mobile phase. The structures of the isolated compounds included: cycloart-25-ene-3 Beta, 24-diol [1], cycloart-23[Z]-ene-3 Beta, 25-diol [2], cycloart-23[E]-ene-3 Beta, 25-diol [3], and 24-methylene-cycloart-3 Beta -ol [4] were elucidated by 13C- and 1H-NMR as well as IR and by the aid of mass fragmentation pattern and comparing with the literature. The biological effects of the compounds were done by the MTT assay on two different cancer cell lines including MDA-MB48 and MCF-7. Among these compounds, cycloart-23[E]-ene-3 Beta, 25-diol [3] was the most active compound on MDA-MB468 cell line [LD50 = 2.05 Micro gmL-1] and cycloart-23[Z]-ene-3 Beta, 25-diol [2] was the most active compound on MCF-7 cell line [LD50 = 5.4 Micro gmL-1]
ABSTRACT
The aim of this study was to evaluate the antibacterial effects of Thymus vulgaris, Thymus caramanicus Zataria multiflora, Zizphora clinopodioides and Zizphora tenuior against four foodborne and four other bacteria including Staphylococcus aureus, Shigella dysenteriae, Salmonella typhimurium, Escherichia coli Staphylococcus epidermidis, Bacillus subtilis, MRSA and Pseiidomona aeruginosa and measuring the amount of total phenolics of the plants. The extracts were prepared by maceration method. Pre-evaluation of the antimicrobial effect was utilized by cup-plate technique and then Minimum Inhibitory Concentration was determined by agar dilution method according to NCCLS. The total phenolics as a possible cause of antibacterial effect, was measured by Folin-Ciocalteucolorimetry. The results showed that T. caramanicus and Z. multiflora were the most effective ones with MIC values between 0.78-3.125 mg/mL against all of the Bactria and Z. tenuior and Z. clinopodioides had the minimum antimicrobial activity. Total phenolic contents of these five plants were different and followed the general pattern of the antimicrobial effect. The antibacterial effects and the total phenolic content of T. caramanicus and Z. multiflora were remarkable and should be investigated more in future studies
ABSTRACT
Achillea tenuifolia Lam. [Asteraceae] afforded a dichloromethane fraction from which three known compounds beta-sitosterol [compound1], 5-hydroxy, 4',6,7- trimethoxy flavone [salvigenin compound 2], and methyl-gallate [compound 3] were isolated for the first time. The structure of isolated compounds was elucidated by different spectroscopic methods. Applying the molar-ratio method, the complexation of salvigenin with Fe [III], Cu[II] and Zn[II], the most abundant type of metal ions in the body, were then evaluated. It was determined that stoichiometric ratio of salvigenin with these cations were as Fe[Salvigenin][2] [H2O][2] and Cu[Salvigenin][2][H2O][2] in methanolic solution without pH control, while zinc ions didn't form significant complexes. The results were confirmed more, by computational molecular modeling of the structure of proposed ligand-complexes by semi-imperical PM3 calculations, which determined negative heat of formation for the complexes Fe[III] and Cu[II] ions as -689.7 and -573.5, respectively and proposed chelating affinity of salvigenin in the following order: Fe[III] > Cu[II] >> Zn[II]
ABSTRACT
Ethanolic crude extract of I. imbricata Lindl. [Iridaceae] was subjected to column chromatography on silica gel with varying portions of MeOH: CHCl[3]. Nigicin [irisolone] was isolated and its identification carried out by IR, UV, MS, 1-D and 2-D NMR spectroscopy
Subject(s)
Isoflavones , Flavonoids , Plant Extracts , Magnetic Resonance SpectroscopyABSTRACT
Two isoflavone, irilin A and irisone B were isolated from Iris songarica and their structures were determined by 1 and 2 D-NMR, IR, UV and MS. The effect of solvent on 1H-NMR spectra of irisone B have been studied