Molecular characterization [Pcr-Based Methods] of Staphylococcus aureus isolated on dogs and cats

A total of 36 isolates of Staphylococcus aureus from hospitalised and out patient dogs and cats were typed by RAPD-PCR, and 36 isolates were selected for further typing by ERIC-PCR and Coagulase gene-PCR and Coagulase gene RFLP, indicating a low degree of polymorphism in the coagulase genes. In this study, it is noticeable that RAPD-PCR displayed desirable typing quality by its ability to group the apparently related isolates from outpatient and hospitalised cats and dogs, whereas ERIC-PCR has the tendency to group the isolates into a single major cluster

SDS page of whole cell protein, immunoblotting and protein A assay for typing of staphylococcus aureus isolated on dogs and cats

A total of 36 isolates of Staphylococcus aureus from hospitalised and out patient dogs and cats were typed using SDS PAGE of whole cell protein, immunoblotting and protein A assayment by ELISA test. 15/24 and 14/24 profiles were recognised using SDS PAGE and immunoblotting respectively. It is concluded that SDS PAGE of whole cell protein and immunoblotting could be used as a typing methods for the characterisation of S. aureus strains. Protein A assayment could be used for the detection of S. aureus strains in samples but could not be used to differentiate between different strains

Detection of plasmids in heavy metal resistance bacteria isolated from the Persian Gulf and enclosed industrial areas

Several heavy metal resistant bacterial strains were isolated from sediment and water samples collected from the Persian Gulf and enclosed industrial areas. All the isolated bacteria were identified by 16S rRNA gene sequencing. Isolated bacteria were tested for the presence of plasmids using the modified alkaline lysate method. The method was effective for identification and characterization of plasmids of different sizes without the use of highly toxic chemicals. The study revealed that the frequency of the occurrence of plasmids in heavy metal resistant bacteria was more than that in the common bacteria. The study also demonstrated that about 66% of isolated bacteria carried large [38-62kb] and/or small sized [4 >/= 2 kb] plasmids. The highest plasmid incidence [84.6%] was detected from industrial wastewater bacteria. A slightly higher incidence of plasmids occurred in bacteria isolated from marine sediments [55.5%] compared to that of the marine water [53.8%]. The findings suggested that plasmids are highly ubiquitous and predominant in most heavy metal resistant bacteria. Removal of lead and cadmium from solution by some of these bacteria was very efficient, approximately 120 mg/g dry weight-as high as 90%. The isolates tested, presented distinct uptake capacities and the best results were obtained for Delftia tsuruhatensis and Pseudomonas AU3411 respectively