Expression of survivin in squamous cell carcinoma and transitional cell carcinoma of the urinary bladder: A comparative immunohistochemical study

PURPOSE: To compare the expression of survivin and its association with clinicopathological criteria in major types of urinary bladder carcinoma, specifically, transitional cell carcinoma with and without squamous differentiation and squamous cell carcinoma. MATERIALS AND METHODS: Immunohistochemical staining for survivin and Ki67 was performed on paraffin-embedded sections of 104 carcinomas: 52 transitional cell carcinoma, 20 transitional cell carcinoma with squamous differentiation, and 32 squamous cell carcinoma. Expression of survivin in >10% of tumor cells was described as altered survivin status. Ki67 staining in >20% of tumor cells was described as a high proliferation index. RESULTS: Altered survivin expression was detected in 60/104 specimens (58%) and was significantly more frequent in transitional cell carcinoma (78%) than in squamous cell carcinoma (38%) or transitional cell carcinoma with squamous differentiation (40%) (p<0.0001). In transitional cell carcinoma but not in squamous cell carcinoma, altered survivin status was associated with higher tumor grade, higher proliferation index, and recurrence. In the whole specimens, altered survivin expression was significantly associated with advanced stage (p<0.001), recurrence (p=0.005), distant metastasis (p<0.001), and death (p=0.001). In the multivariate analysis, altered survivin was an independent poor prognostic factor for recurrence. CONCLUSIONS: Unlike in transitional cell carcinoma, alteration of survivin expression in squamous cell carcinoma occurs less frequently and is not associated with features of tumor aggression or patient outcome. These findings raise a question: are urinary bladder carcinoma patients with squamous cell carcinoma type suitable candidates for survivin vaccine? This is an important question to be answered before approving the vaccine in management.

Possible protective effect of camellia sinensis [green tea] against acrylamide induced testicular damage in albino rats

Acrylamide is a proved toxin for testicular function, found in food when heated for long period of time. Green tea [Camellia sinensis] is a potent antioxidant; the aim of this study was to investigate the protective effect of green tea extract against the toxic effects of acrylamide in rat testes. acrylamide was administered orally by gastric gavage to rats in different doses and also the extract of green tea was administered orally to different groups of animals in combination with the acrylamide. The weight of animals, testosterone hormone level and histopathological effects upon testicles were evaluated. Testosterone hormone level in serum was significantly decreased in those with acrylamide toxicity either in low or high dose. The histopatological findings were in the form of thickening of the tubuler epithelium and degenerations of germ cells. All findings significantly improved with the co administration of green tea extract with the acrylamide. Green tea extract reversed all the toxic effects of acrylamide even in high dose for long period [90 days]. green tea extract is a potent antioxidant antidote for the acrylamide toxic effects upon testicular function

Role of apoptosis proteins survivin, BAX and BCL-XL in laryngeal squamous cell carcinoma progression

We used immunohistochemistry to investigate a potential role of SVV as an early predictor of malignant transformation in precancerous and cancerous lesions of the larynx, we also sought to examine the expression of Bcl-X[L] and Bax in laryngeal SCC analyze the relationships between their expression and prognostic factors including site, histological grade, clinical staging and lymph node metastasis. This study included 6 normal laryngeal mucosae, 7 dysplastic laryngeal epithelia, 5 in situ laryngeal carcinomas, and 32 hiopsied laryngeal SCC. Clinical evaluation was done. Specimens were forma I in-fixed, paraffin-embedded, stained with H and E, classified and graded according to WHO classification, [2005] and immunostained to detect Survivin and Bcl-X[L]. and Bax proteins using the avidinbiotin peroxidase method. Survivin expression gradually increased significantly 'with advance of laryngeal carcinoma through the sequence of dysplasia, in situ carcinoma and infiltrating carcinoma [P < 0.04]. There is a statistically significant increase in Survivin expression with increasing tumor grade [P < 0.03] and with advance in clinical staging [P < 0.01]. Regarding lymph node metastasis SVV was more expressed in laryngeal SCC exhibiting lymph node metastasis [P < 0.02]. Bax expression was decreased significantly in infiltrating laryngeal carcinoma [P < 0.04] compared with premalignant lesions of the larynx. In contrast, Bcl-X[L] was increased significantly with the advance of laryngeal. carcinoma through dysplasia, carcinoma sequence [P < 0.02]. There was no statistically significant difference in either Bax or Bcl-X[L] expression considering tumor differentiation, advanced clinical staging or lymph node metastasis. Survivin plays cm important role in the initiation of laryngeal cancer and its progression towards higher grades, invasion and metastasis. Bax and Bcl-X[L] play their role early in cancer initiation but have nothing to do as the tumor progresses to higher grades, infiltrates deeply, or giving lymph node metastasis