ABSTRACT
To find the cure of world's one of the leading morbid and mortal disorders; diabetes mellitus and its most prevalent complication, 'diabetic-dyslipidemia', is one of the leading health challenges of 21st century. The use of phytomedicine is a glimmer of hope in this scenario. Studies of current decade have shown that methanolic extracts of Zingiber officinale and Curcuma longa have highly effective therapeutic potentials against the aforesaid disorders, however, which of the extracts has more potential is still unclear. Furthermore, synergistic effect of the extracts has never been studied. Forty-eight Albino adult rats of either sex were randomly divided into eight groups. A-D groups were containing healthy rats while E-H groups were of induced diabetic-dyslipidemic rats. For forty-two days, rats of each group were given either distilled water or Zingiber officinale methanolic extract [ZOME] or Curcuma longa methanolic extract [CLME] or ZOME+CLME therapies at dose rate of 300mg/100 mL dist. H2O/kg body wt/day. FPG and lipid profiles were estimated before and after the trial, and were statistically analyzed by one-way ANOVA along with Posthoc Tukey's multiple comparison tests. Although, ZOME and CLME significantly [P<0.05] lowered fasting plasma glucose [FPG] levels and controlled lipid profiles in diabetic-dyslipidemic rats; yet, synergistic therapy of both extracts [ZOME+CLME] most significantly [P<0.05] controlled all parameters of diabetic-dyslipidemia [78.00 +/- 1.06mg/dL FPG, 62.00 +/- 0.58mg/dL TG, 66.50 +/- 0.76mg/dL cholesterol, 32.00 +/- 0.36mg/dL HDL, 22.43 +/- 0.64 mg/dL LDL, and 12.40 +/- 0.12mg/dL VLDL]. Our findings may be useful to formulate new medicines having multiple potentials to control diabetes mellitus, dyslipidemia, and diabetic-dyslipidemia
ABSTRACT
Lysine executes imperative structural and functional roles in body and its supplementation in diet beneficial to prevent the escalating threat of protein deficiency. The physical mutagenesis offers new fascinating avenues of research for overproduction of lysine through surplus carbohydrate containing agriculture waste especially in developing countries. The current study was aimed to investigate the potential of UV mutated strain ofBrevibacterium flavum at 254 nm for lysine production. The physical and nutritional parameters were optimized and maximum lysine production was observed with molasses [4% substrate water ratio]. Moreover, supplementation of culture medium with metal cations [i.e. 0.4% CaSO[4], 0.3% NaCl, 0.3% KH[2]PO[4], 0.4% MgSO[4], and 0.2% [NH[4]][2]SO[4] w/v] together with 0.75% v/v corn steep liquor significantly inhanced the lysine production up to 26.71 +/- 0.31g/L. Though, concentrations of urea, ammonium nitrate and yeast sludge did not exhibit any profound effect on lysine production. Biological evaluation of lysine enriched biomass in terms of weight gain and feed conversion ratio reflected non-significant difference for experimental and control [+ve] groups. Conclusively, lysine produced in the form of biomass was compatible to market lysine in its effectiveness and have potential to utilize at commercial scale