ABSTRACT
Background:Practice of hand washing is very important to eliminate the microbial contamination especially during the work in laboratories, hospital and even at home before taking food. Proper use of hand sanitizer can significantly reduce the rate of hospital acquired infection also. Methods:The current investigation was designated to identify different bacterial species from the upper skin of hands of the laboratory managements through conventional culture methodsand the efficacy of the samples (Dettol, Purell and Savlon) against the isolated bacteria throughagar well diffusion method and minimum inhibitory concentration (MIC)Results:Different concentrationssuch as 25%, 50% and100% of eachof antimicrobial agentsshowed their antibacterial activity againstStaphylococcus spp., Klebsiellaspp.,E. coliand Pseudomonasspp. those were isolated from the hand.25% of Dettolexhibited 20mm zone diameter against Klebsiellaspp. Whereas 25% of Purell and Savlon unveiled 15mm and 22mm zone of inhibition against E. coliand Pseudomonasspp. respectively. In case of 100% sample of Dettol, Purelland Savlon, the highest zone diameter was observedas 55mm, 50mm and45mm against Klebsiellaspp.,Staphylococcus spp., and E. coliconsecutively. The MIC of Dettol sample was 8μl against Staphylococcus spp., and Pseudomonasspp.In case of Purell, the MIC was 128μl againstStaphylococcusspp., Klebsiellaspp. and Pseudomonas spp. were inhibited at 4μl samples.Conclusions:The in-vitro antibacterial activity of the hand sanitizers was so satisfactory against the isolated bacteria. This finding would be very helpful for the laboratory management in order to minimize the rate of contamination during the research and supervision of the different experiment.
ABSTRACT
Aims: Present study endeavored to examine the growth and survival of microorganisms within 6 categories of oral herbal medicines commonly used by the community within Dhaka metropolis. Methodology: Samples were analyzed for the presence of bacteria and fungi up to 14 days. The microbial analysis was conducted by conventional cultural and biochemical methods. The in vitro anti-bacterial activity of the medicines was also detected employing agar well diffusion method. Results: Initially all samples were found to be contaminated with total viable bacteria (102-104cfu/ml); however, the fungal and pathogenic growth was not observed. In course of time, the bacterial and fungal load increased up to 106cfu/ml and 103cfu/ml, respectively in most of the samples up to 14 days. The staphylococcal growth commenced after 48 hours in all samples and vigorously increased in two samples up to 105cfu/ml. Two categories of samples were found to be populated with Klebsiella spp. (102cfu/ ml); while other pathogenic bacteria were completely absent. Out of 6 categories of samples tested, 4 were found to exhibit the anti-bacterial trait against a few bacteria examined. Significant activity was found for sample 1 against E. coli, and sample 3 against E. coli and Staphylococcus spp. Sample 2 exhibited moderate activity against 4 test bacteria; while sample 4 was also noticed to be moderately active against 2 test bacteria. Conclusion: Overall, together with the trivial anti-bacterial features, the appearance of massive bacteria and fungi after 14 days in most of the samples with an excessive staphylococcal load may pose the probable health risks to the medicine users.