ABSTRACT
Ajisamat, an herb commonly used as an aphrodisiac in the Malaysian traditional medicine, corresponds to two different species from different families - Salacia macrophylla Blume, Celastraceae, and Prismatomeris glabra (Korth.) Valeton, Rubiaceae. Macromorphological inspection of the vegetative parts both plants reveals only a slight difference in the arrangement of the petioles. Microscopic investigation of the plants roots used as crude drugs revealed however distinctive anatomical features. Prismatic calcium oxalate crystals and banded paratracheal parenchyma are characteristics of S. macrophylla while P. glabra displays an abundance as crystals. Other features such as vessels diameters and arrangements are also of diagnostic importance. Some of these characters were also identified in the powder of thes e plant materials and proposed for diagnostic purpose. The values for extraction of ethanol and water as well as total ash, acid-insoluble ash, water-soluble ash and sulfated ash were determined for both plants. Phytochemical studies were carried out on hexane and chloroform extracts of S. macrophylla and methanolic extract of P. glabra. S. macrophylla was shown to contain highly oxidized pentacyclic triterpenes while P. glabra contains anthraquinones. The pharmacognostical and hytochemical information can be utilised as the identification tools for Salacia macrophylla and Prismatomeris glabra .
ABSTRACT
Evaluation of amino and fatty acids compositions in Haruan Traditional Extracts (HTE) was done using HPLC and GC methods. The HTE contained at least 17 amino acids with glutamic acid, glycine, leusine, aspartic acid, proline, alanine and arginine are the most, with values 1.87 - 43.13 mg/g, 21.80 - 80.85 mg/g, 7.85- 40.19 mg/g, 13.85 - 44.07 mg/g, 9.49 - 45.46 mg/g, 11.38 - 35.25 mg/g and 5.99 - 21.79 mg/g, respectively. Meanwhile, the highest percentage of fatty acids is palmitic acid; 3.54 - 26.84 percent of total protein. The others major fatty acids are stearic acid, oleic acid and linoleic acid with values 3.25 - 15.90 percent, 1.40 - 27.68 percent, 0.51 - 7.82 percent of total protein, respectively. HTE also found to have 4 extra bioactive compounds labelled as 1 to 4 on chromatographic tracing which in line with previously finding. It is concluded that the HTE is containing all the important amino acids plus some fatty acids, which is the basis to conduct antioxidant composition in both fresh Haruan and the HTE which was claimed to have wound healing properties. Comparative study was also carried out in various other extraction protocols, including commercial product.
Evaluación de las composiciones de aminoácidos y ácidos grasos en Haruan Extractos tradicional (HTE) se realizó mediante métodos de HPLC y GC. La HTE contenía al menos 17 aminoácidos con ácido glutámico, glicina, leucina, ácido aspártico, prolina, alanina y arginina como mayoritarios, con valores de 1.87 - 43.13 mg/g, 21.80 - 80.85 mg/g, 7.85 - 40.19 mg/g, 13.85 - 44.07 mg/g, 9.49 - 45.46 mg/g, 11.38 - 35.25 mg/g and 5.99 - 21.79 mg/g, respectivamente. Mientras tanto, el mayor porcentaje de ácidos grasos es el ácido palmítico; 3.54 - 26.84 por ciento de la proteína total. Otros ácidos grasos importantes son el ácido esteárico, ácido oleico y ácido linoleico con valores de 3.25 - 15.90 por ciento, 1.40 - 27.68 %, 0.51 - 7.82 por ciento de la proteína total, respectivamente. HTE también encontró cuatro compuestos bioactivos adicionales etiquetados de 1 a 4 en el seguimiento cromatográfico que está de acuerdo con resultados previos. Se concluye que la HTE contiene todos los aminoácidos importantes además de algunos ácidos grasos, que es la base para llevar a cabo la composición antioxidante, tanto en fresco Haruan y la HTE que se afirma poseen propiedades curativas. Estudios comparativos se llevaron a cabo con otros protocolos de extracción, incluido el producto comercial.