Bioconversion of agriculture waste to lysine with UV mutated strain of brevibacterium flavum and its biological evaluation in broiler chicks

Lysine executes imperative structural and functional roles in body and its supplementation in diet beneficial to prevent the escalating threat of protein deficiency. The physical mutagenesis offers new fascinating avenues of research for overproduction of lysine through surplus carbohydrate containing agriculture waste especially in developing countries. The current study was aimed to investigate the potential of UV mutated strain ofBrevibacterium flavum at 254 nm for lysine production. The physical and nutritional parameters were optimized and maximum lysine production was observed with molasses [4% substrate water ratio]. Moreover, supplementation of culture medium with metal cations [i.e. 0.4% CaSO[4], 0.3% NaCl, 0.3% KH[2]PO[4], 0.4% MgSO[4], and 0.2% [NH[4]][2]SO[4] w/v] together with 0.75% v/v corn steep liquor significantly inhanced the lysine production up to 26.71 +/- 0.31g/L. Though, concentrations of urea, ammonium nitrate and yeast sludge did not exhibit any profound effect on lysine production. Biological evaluation of lysine enriched biomass in terms of weight gain and feed conversion ratio reflected non-significant difference for experimental and control [+ve] groups. Conclusively, lysine produced in the form of biomass was compatible to market lysine in its effectiveness and have potential to utilize at commercial scale

Antimicrobial activities of three species of family mimosaceae

The antimicrobial activities of crude methanolic extract of leaves of Acacia nilotica L., Albizia lebbeck L. and Mimosa himalayana Gamble belonging to family mimosaceae were investigated in this research work. Antibacterial activity was studied by agar well diffusion method against one gram-positive Bacillus subtilis and three gram-negative Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumonia. Crude extract of all plants showed best activity against gram-negative bacterial strains while minor inhibition zones were found against gram positive bacterial strains. Antifungal activity of crude plant extract was screened by agar tube dilution method against Aspergillus nigar and Aspergillus flavus. These results showed that these plants extracts have potential against bacterias, while against fungi their activity is not much effective