ABSTRACT
Thirty semen specimens included in the study were taken from non-smokers and another thirty specimens from heavy smokers. Each specimen was divided into two portions. One portion was centrifuged and the pellet was resuspended in an equal volume of S.P. from the opposite participant group [i.e. spermatozoa from a non-smoker into S.P. of a smoker and vice versa]. The other portion remained without processing. Both portions were tested for sperm acrosin activity by gelatin substrate film technique. Scoring the diameters of the haloes around sperm heads did evaluation of the acrosin activity. Before processing, the haloes of the nonsmokers were larger than those of smokers indicating that their acrosin activity is higher. After processing, the haloes around the heads of spermatozoa in nonsmoker group specimens incubated in S.P. of smokers became smaller than before processing indicating that their acrosin activity became lower. On the other hand, haloes around the heads of spermatozoa in smoker group specimens incubated in S.P. of nonsmokers became larger than before processing.These effects of S.P. from both groups on acrosin activity have added to the detrimental effects of smokers S.P. on nonsmokers spermatozoa