ABSTRACT
Investigations were carried out on the effects of pesticide Chlorpyrifos [organophosphate] on skin of adult frogs of Euphlyctis cyanoplyctis. The effects of pesticide were observed on frog's skin during 90 days after the treatment. Study of skin cells was carried out by sectioning of fixed tissue by Paraffin Section Technique. The results obtained in the long-term experiment showed that under the effects of chlorpyrifos sections examined reveal skin covered fibrocollagenous tissue exhibiting areas of necrosis and aggregate of mild chronic inflammation, pigment containing basal keratinocytes were identified in epidermis. Variable size measured fibers with degenerated and regenerative changes also noted. A few fiber stain internalization took place in collection of chronic and non stapile inflammatory cells along with tissue necrosis. Chronic granulomatous inflamation is not identified. However, histolytic collision has been found. No evidence of malignancy was observed