Alleles for Associated with Fy(a), Fy(b), Fy(x) and Fy Antigens in a Korean Population / 대한수혈학회지

BACKGROUND: In the Duffy blood group system (Fy(a), Fy(b), Fy(x), and Fy antigens), Fy(x) antigen is associated with weak Fy(b) while Fy antigen means the null phenotype Fy (a-b-). Fyx antigen and Fy antigen result from the polymorphisms of Fy125 allele. This report assessed the allele frequency and genotype frequency of Fy(a), Fy(b), Fy(x), and Fy antigens in Koreans. METHODS: We performed a study of the followings on 253 visitors to the health promotion center of Seoul National University Bundang Hospital: PCR-RFLP and PCR-SSP for the detection of Duffy 125G > A and -33T > C; PCR-SSP for the detection of Duffy 265C > T and 298G > A. RESULTS: The results of PCR-RFLP and PCR-SSP were consistent with each other in a total of 253 subjects. Allele frequency was as follows: Fy 92.3%, Fy(125) 6.1%, and fy(125/265) 1.6%. The fy(125/265) allele was newly observed. Fy(125/298), fy(125/265/298), and fy(-33/125) alleles were not detected in Koreans. The distribution of Duffy phenotypes in Koreans was as follows: Fy (a+b-) 88.1%, Fy (a-b+) 0.4%, Fy (a+b+) 11.5%, and Fy (a-b-) 0.0%. Fy (a+) was 99.6% and Fy (b+) was 11.9%. CONCLUSION: In our study for Duffy polymorphisms, the frequency of Fy allele was very high. The frequency was similar to those of other Asian populations, but different from those of Caucasians. The fy(125/265) allele, which was associated with Fy(x) antigen, was newly detected in Koreans.

Genotype and allele frequencies of the platelet glycoprotein genes associated with arterial thrombosis in Korean population / 대한수혈학회지

BACKGROUND: Ischemic heart disease and cerebrovascular disease are the main causes of death and platelets are responsible for the formation of arterial thrombi. Platelet membrane glycoproteins (GP) associated with coagulation pathway are GPIb/V/IX, GPIa/IIa, and GPIIb/IIIa. We evaluated genotype and allele frequencies of seven platelet glycoprotein genes associated with arterial thrombosis. METHODS: Genomic DNA was isolated from peripheral blood of 300 unrelated Korean and single nucleotide polymorphism of platelet glycoproteins was analyzed. PCR with sequence specific primers was used to investigate GPIa C807T and GPIbalpha VNTR polymorphism. PCR-RFLP (restriction fragment length polymorphism) was used to investigate GPIa G1648A and C2531T, GPIbalpha C524T and T-5C, and GPIIIa T1565C polymorphism. RESULTS: The allele frequencies of GPIa C807T were 807C 0.733, 807T 0.267; GPIa 1648G 0.975, 1648A 0.025; GPIa C2531T, 2531C 1.000, 2531T 0.000; GPIbalpha C524T, 524C 0.927, 524T 0.073; GPIbalpha VNTR, A 0.017, B 0.015, C 0.558, D 0.410; GPIbalpha T-5C, -5T 0.726, -5C 0.274; GPIIIa T1565C, 1565T 0.995, 1565C 0.005. CONCLUSION: The genotype and allele frequencies of GPIa G1648A, GPIbalpha C524T, and GPIIIa T1565C were similar to established data. GPIa 807T and -5T allele of Kozak polymorphism showed low frequency compared with other ethnic group. Allele frequencies of GPIbalpha VNTR A and B alleles were very alike (0.017 vs 0.015). In this study, we firstly evaluated the genotype and allele frequencies of GPIa C2531T and GPIbalpha VNTR, T-5C polymorphisms in Korean population. This study will serve as a basic data for the study of platelet glycoproteins associated with arterial thrombosis in Korean.

Detection of Helicobacter pylori and BabA (Blood-group Antigen Binding Adhesin) in Saliva and Gastric Tissue by Polymerase Chain Reaction / 임상검사와정도관리

BACKGROUND: Saliva is considered an important vector for the Helicobacter pylori infection. The presence of the babA2 gene, encoding for BabA (blood-group antigen binding adhesin), in the H. pylori genome is crucial for H. pylori-related pathogenesis. METHODS: The study was performed in the group of 215 patients. The detection of H. pylori and babA2 in saliva and gastric tissue was done by PCR (polymerase chain reaction). Moreover, gastric tissues were stained with hematoxylin-eosin as well as with modified Giemsa methods for the analysis of Helicobacter pylori density. RESULTS: The positive rate of H. pylori by nested PCR was 78.6% in gastric tissue and 72.7% in saliva. In addition, the positive rate of H. pylori was 55.5% by the histological analysis of Helicobacter pylori density in gastric tissue. The positive rate of babA2 by PCR was 33.9% in gastric tissue, and 8.2% in saliva. CONCLUSION: We revealed that the H. pylori PCR results obtained in gastric tissue correlated well with those obtained in saliva. As saliva is more available specimen, it is more suitable for clinical application of H. pylori detection by PCR. However, clinical use of - BabA PCR seems to be limited because of its low-sensitivity.