ABSTRACT
Background: Mutations in the coding region of the Chemokine Receptor 5 [CCR5] genes reduce or eliminate CCR5 expression in immune cells and progression of HCV infection. This study aimed to investigate the role of this mutation in HCV infection in Iranian patients in comparison with healthy individuals
Methods: 100 HCV infected patients and 100 healthy individuals were randomly selected. The CCR5-DELTA32 genotypes were determined using specific primers and PCR method
Results: The agarose gel electrophoresis showed a189-bp fragment from wild type for both alleles of CCR5 gene. The CCR5-DELTA32 allele was not found in any HCV infected and healthy subjects
Conclusion: The mutation in CCR5 gene was not detected in any of the two groups; therefore, the role of CCR5 gene expression in immune cells and progression of HCV infection needs to be studied in larger samples in our country
ABSTRACT
Background: Epidemiological studies have probed the correlation between telomere length and the risk of lung cancer, but their findings are inconsistent in this regard. The present meta-analysis study has been carried out to demonstrate the association between relative telomere length in peripheral blood leukocytes and the risk of lung cancer using an established Q-PCR technique
Methods: A systematic search was carried out using PubMed, EMBASE, and ISI before 2015. A total of 2925 cases of lung cancer and 2931 controls from 9 studies were employed to probe the relationship between lung cancer and telomere length .ORs were used at 95% CI. Random-effects models were used to investigate this relationship based on the heterogeneity test. Heterogeneity among studies was analyzed employing subgroup analysis based on type studies and the year of publication
Results: Random-effects meta-analysis revealed that patients with lung cancer were expected to have shorter telomere length than the control [1.13, 95% CI: 0.82-1.81, P=0.46]. The summary of the pooled ORs of telomere length in adenocarcinoma lung cancer patients was 1 [95%CI=0.68-1.47, I2=93%] compared to patients with squamous cell lung cancer, which was 1.78 [95% CI=1.25-2.53, I2=3.9%]. The meta-regression revealed that the effect of telomere length shortening, decreased and increased with the year of publication and the age of risks to lung cancer, was clearly related to short telomeres lengths
Conclusion: Lung cancer risks clearly related with short telomeres lengths. In patients with breathing problems, lung cancer risk can be predicted by telomere length adjustment with age, sex, and smoking