ABSTRACT
To study the effects of Ketoconazole induced liver damage, compare with control group and correlate with previous studies. Forty adult male albino rats were used for this study. Group-A served as control animals, received injection of normal saline in dose of 0.05 ml/100 gm of body weight intraperitoneally daily for 03, 07, 15 and 30 days. Group-B received injection of Ketoconazole 40 mg/kg of body weight intraperitoneally daily for 03, 07, 15 and 30 days of treatment. Animals were sacrificed after completion of treatment under ether anaesthesia. Liver were removed, fixed in 10% and alcoholic formalin for 24-48 hours. They were dehydrated in ascending strength of alcohol and paraffin tissue blocks were made 5 IJm thick section were stained with H and E for general morphology and micrometry and Gomori's reticulin stain for observing reticulin fibres. The results were compared with control. Analyzed statistically with student 't' test and correlate with previous studies. Ketoconazole treated animals showed distortion of hepatic architecture, increase size of hepatocytes, decrease nuclear diameter and necrosis of hepatocytes. Increased amount of reticulin fibres and area of focal fibrosis within hepatic lobule as compared to control group-A animals. It is concluded from this study that Ketoconazole induce injury is dose and duration of therapy dependent and due to its cost effective frequent use needs further research in humans