ABSTRACT
Aim: In present study we have elucidated the role of 2758 A>G [rs696], in the recognition site of miR449a in the 3' UTR of NFKB inhibitor alpha [NFKBIA] gene, in development of sporadic colorectal cancer
Background: Colorectal cancer [CRC] is rated as second cause of cancer death. Genetic determinants are considered as driving forces in development of sporadic CRC. Single nucleotide polymorphisms [SNPs], are attributed as the main genetic factor in cancers susceptibility. MicroRNAs, are key players in post-translational gene regulation by binding to their specific recognition sequences located at 3' untranslated region [UTR] of mRNAs
Methods: A case.control study using 143 CRC patients and 137 noncancerous counterparts were undertaken in order to determine rs696 genotypes using polymerase chain reaction. restriction fragment length polymorphism [PCR.RFLP] method
Results: There was a significant difference for the genotype frequencies of rs696 between patients and controls. The frequencies of GG, AG, AA genotypes in the control group were 38.7, 45.3, and 16.1 %, respectively, and the genotype frequencies in case group were 19.6, 40.6, and 39.9 %, respectively
Conclusion: Our results suggest significant correlation between rs696 polymorphism and colorectal cancer risk