ABSTRACT
Background: cross-contamination between cells is a usual mistake at cell culture laboratories and cell banks worldwide. MRC5 diploid cell and Rk-13, Vero and Hela continuous cell lines are used in different stages of human viral vaccines propagation at Razi Vaccine and Serum Research Institute of Iran. However diploid and continuous cells are propagated at separated cell culture laboratory and continuous cells can contaminate MRC5 diploid cells. Therefore, a sensitive test is needed. World Health Organization recommends few molecular and cellular techniques to cell characterization
Materials and Methods: the present study was therefore designed to set up an indirect immunofluorescence [IIF] test as follows: Polyclonal anti-MRC5 cell and anti-rabbit antibodies were prepared in rabbit and goat, respectability. Anti-rabbit IgG was purified using protein G affinity chromatography, conjugated to fluorescein isothiocyanate [FITC], and then further purified to remove unbound FITC using Sephadex G 25 chromatography. Using double immunodiffusion assay, purification of homemade anti-rabbit IgG was asssayed by observation of a single arch
Results: the titer of homemade FITC conjugated goat anti rabbit IgG was measured 1/16 vs 1/8 of commercial type. Fluorescein/protein molar ratio of local made fluorescein goat anti-rabbit IgG was measured 3.44 and its protein concentration and FITC concentration were determined 1.198 mg/ml and 0.01 mg/ml, respectively
Conclusion: moreover, homemade IIF test showed 100% intra-laboratory reproducibility. Purity of three batches of MRC5 working seed cell was verified using in- house IIF test and no contamination to continuous cell lines was found
ABSTRACT
Currently, the scientific output indexed in databases such as ISI is the major criteria for evaluation and ranking of countries, researchers, institutes and universities in the world. Each country's share in the world's scientific output in general and specifically in a specific field will be evaluated accordingly. Therefore, determination of the rate of growth of articles from Iranian authors in the ISI database in the field of immunology, its distribution and growth in different years, average citation per paper in comparison with the Iranian and world average, co-authorship, the most cited papers, the most prolific authors, the most prolific Iranian universities and institutions, are the main objectives of the study. The study area is scientometrics and the necessity of the survey methods and citation analysis has been used. Findings show that from 1974 to the end of 2010 a total of 1775 articles of Iranian writers in the immunology field have entered into ISI Web of Science. According to the results, the number of articles has been high in the recent years. Most collaboration of the Iranian authors has been with their counterparts from USA. Immunology articles have had the most interaction with the field of transplantation. Most of the articles of Iranian scholars in the field of immunology are published in the Transplantation Proceedings journal. Tehran University of Medical Sciences [TUMS] is the most active center and Ardeshir Ghavamzadeh is the most prolific author. The costs of 10.99% articles is provided by Iranian and foreign universities and institutes. Iranian papers in the field of immunology have steadily increased and in the recent years have reached its peak. Iranian immunologists have disposition to co-authorship with their counterparts from other countries which is almost the same with other subject areas in Iran. The average citation per paper in the field of immunology is higher than that of the Iranian average and lowest than the global average, much lower than global and Middle East average in the field of immunology. Tehran University of Medical Sciences is the most prolific university. Also, the most prolific authors are affiliated to the TUMS. Domestic and foreign universities and institutions in financing research have had little share
ABSTRACT
Currently, share of the scientific output, citation per paper, and co-authorship for articles indexed in databases such as ISI Web of Science, are very important criteria for the evaluation and ranking of countries, researchers, institutes, articles, disciplines and journals in the world. Therefore, the main objectives of the study were to determine co-authorship, the average citation per paper and the most prolific Iranian university or institution in the field of immunology. For performing this scientometric study, we employed survey methods and citation analysis of the indexed papers in the ISI Web of Science from 1974 to 2010. A total of 1775 articles by Iranian authors in the field of immunology had been indexed in the database for the aforesaid period. Most collaborations and co-authorship of Iranian authors was with their counterparts from the USA. The average citation per paper in the field was 6.26 per paper. Tehran University of Medical Sciences had the highest number of articles in the database. According to the results, Iranians collaborated with American peers more than any other nationality andthe average citation per paper in the field of immunology is higher than that of other fields in Iran but lower than the global average. Tehran University of Medical Sciences is the most prolific university in the field