ABSTRACT
Background: Glyphosate [N-phosphonomethyl Glycine] is an organophosphorus pesticide with dangerous effects on the environment. In this study, the biodegradation of glyphosate herbicide by halophilic bacteria isolated from Qom Hoze-Soltan Lake has been investigated
Methods: After sampling and bacterial isolation, native halophilic strains grown in the presence of glyphosate at a wavelength of 660 nm and also the disappearance of the glyphosate in the plates at a wavelength of 220 nm were determined and the dominant bacteria were isolated. Biochemical, molecular [according to the 16S rRNA sequence], antibiotic, and the Minimum Inhibitory Concentration [MIC] test was performed for the dominant bacteria. Analysis of the remaining glyphosate herbicide was performed by HPLC analysis after derivation with FMOC-Cl
Results: According to the results of the biochemical, antibiotic and molecular 16S rRNA tests, the native halophilic isolates with the ability to biodegrade glyphosate were gram positive cocci very similar to Salinicoccus spp. The results of HPLC showed that Salinicoccus spp is able to biodegrade glyphosate herbicide
Conclusion: The native bacteria in Qom Hoze-soltan lake, Iran can be used for biodegradation of glyphosate herbicide
ABSTRACT
Vibrio species have been known as the cause of gastrointestinal and extraintestinal disorders, which usually appear epidemically in some seasons of the year. Since the sea has been known as one of the important ecosystems of Vibrio species, this study was carried out with the aim of determining the prevalence of Vibrio species and their microbial resistance in different coastal regions of the south of country in different seasons of year. In this descriptive cross-sectional study, 600 water samples taken from the coastal waters of Bandar Abbas were evaluated in terms of presence of Vibrio strains in two seasons of spring and winter. For primary detection, alkaline peptone water and TCBS were used, and the final identification was carried out using biochemical tests [oxidase, motility, Indole ring, KIA, and other biochemical tests such as 0% NaCl, 6% NaCl, VP, and ONPG]. V. harveyi [2.3%], V. cholerae [1.7%], V. parahaemolyticus [1.3%], V. furnissii [1.2%], V. metschnikovii [1%], P. shigelloides [1%], A. hydrophila [0.5%], V. vulnificus and V. mimicus [0.3%], and V. fluvialis [0.2%] had the highest prevalence. Also, in terms of the studied seasons, 71.2% of the contamination was related to winter season and 28.8% to spring season, which showed that the cases of contamination in winter was higher than in spring. Based on the study of researchers, the isolation of these species increases after rain. In the present study, this parameter had a positive effect on the isolation of Vibrio species.