ABSTRACT
Aims: This research investigated the thrombolytic, cytotoxic and antidiabetic effects of Paederia foetida leaf methanol extract. Place and Duration of Study: Department of Genetic Engineering and Biotechnology and Department of Biochemistry and Molecular Biology, University of Chittagong, Bangladesh, between April to December, 2012. Methodology: An in-vitro thrombolytic model was used to check the thrombolytic effect of Paederia foetida extract using streptokinase as a positive control. Cytotoxic effect was measured by Brine shrimp lethality bioassay. Antidiabetic effect of the extract was conducted in alloxan induced diabetic model of Swiss albino mice. Data were analyzed by one way ANOVA using statistical package for social science (SPSS) software. Results: In in-vitro thrombolysis, Paederia foetida extract (100μl) lysed 21.40 ± 1.39 %, Streptokinase (positive control) 81.42 ± 0.88 % and water (negative control) 4.63 ± 0.31% of the blood clots. These clot lysis values were statistically different (p<0.05) from each other. In Brine shrimp bioassay, lethal concentration (LC50) of Paederia foetida extract was found 65.31 μg/mL which was statistically significant compared to Vincristine sulfate (positive control, LC50=0.76 μg/mL). The extract showed a significant and dosedependent decrease in blood glucose level in alloxan induced diabetic mice. The effect of the extract was comparable to the reference antidiabetic drug metformin (150 mg/kg BW). Conclusion: The results demonstrated a moderate thrombolytic, cytotoxic and antidiabetic effect of Paederia foetida leaf extract to be analyzed further for prospective pharmaceutical preparation.
ABSTRACT
Cassia alata is a pan tropical shrub. It has been reported to have medicinal activities like laxative effect and active against ringworm, skin diseases and ulcer. The leaf extract of this plant has shown antibacterial activity. In this study, seed oil extracted from Cassia alata , was studied for Antibacterial, Thrombolytic and Cytotoxic activity by using in vitro techniques. Chloroform extract of the seed oil was tested for Antibacterial activity using disc diffusion method and that extract showed potent antibacterial activity against both Gram(+) and Gram(-) bacteria. DMSO extract of the seed oil was tested for Brine shrimp lethality bioassay using Brine shrimp nauplii. The LC50 of DMSO extract of Cassia alata seed oil was found to be 250μg/ml, indicates that the oil has moderate pharmacological action. The DMSO extract of the seed oil was also tested for in vitro thrombolytic activity. The extract showed reasonable thrombolytic activity against negative control (water).Further investigation on the plant is required to confirm their pharmacological activity and thereby utilizing them as useful medicinal plant.