ABSTRACT
Background EBV was the first human virus directly implicated in carcinogenesis. Since its discovery it has been considered as a major player in the development of a wide range of cancers. Recent studies have concluded EBV itself or infected cell clones might promote replication of the HCV
EBNAl is responsible for supporting HCV replication, suggesting that EBV may be involved in the development of hepatocellular carcinoma [HCC] The detection of EBV gene products in HCC additionally supports this assumption
Methods Methods This study was carried out on cases divided into three groups:-Group I included 15 very low cone, of EBV [IgG] with both negative HCC and HCV RNA patients, Group 2 included 15 moderate cone, of EBV [IgG] with positive HCC and negative HCV RNA patients and Group 3 included 15 high cone, of EBV [IgG] with both positive HCC and HCV RNA patients Reverse transcription PCR [RT-PCR] was performed to detect HCV RNA
Immunohistochemistry was performed to detect EBV[IgG].. The positive ratios were compared between HCC group and control group
Results Our retrospective study determines serum level of hepatitis C, Epstien - Barr [IgG] and Alpha feto protein and to correlate their serum levels with each other in the patient of hepatocellular carcinoma assessed by histopathological staging of liver biopsy. HCV level increased significantly with progression of Serum level ofEBV[IgG] and AFP had no statistical significant change with EBV[IgG]
Conclusion: The existence of EBV infection in HCC tissues suggests that EBV may be involved in the hepatocellular carcinogenesis in Egypt. HCV infection may be a major cause of HCC. There is correlation between EBV and HCV in the development of HCC. EBV enhancement the replication of HCV