ABSTRACT
Background: proteus bacilli are more commonly associated with UTIs in those individuals with structural or functional abnormalities, especially ascending infections in patients undergoing urinary catheterization
Aim of the study: to estimate the frequency of proteus infection in patients with I urinary tract infections, detection of sensitivity profile to antibiotics and detection of ESBL
Material and methods: one hundred and fifty five patients with symptoms of urinary tract infection were recruited and urine sample from each patient was collected as well as demographic data and associated I risk factors were recorded. Urine culture, antimicrobial susceptibility test, screening and confirmatory tests for detection of ESBL were done
Results and conclusion: the mean age +/- SD was 45 +/- 17 years, 139 were males and 16 were females. Sixty eight patients were catheterized and 87 patients were non-catheterized. E coli was the highest bacterial organism [35%] detected by urine culture among catheterized patients, while Kpneumoniae was the highest among non-catheterized patients. There was a significantly higher percent of proteus among non-catheterized patients than catheterized patients. Proteus alone or proteus with other bacterial infection was detected in 14.8% in catheterized versus 6.1% in non-catheterized patients. Only 13 cases out of 155 cases [8.4%] had positive culture for proteus organism. All are males and most of them were catheterized and had benign enlarged prostate [8 cases; 61%]. Out of 13 Proteus isolates, nine Proteus isolates showed reduced susceptibility to the Ceftazidime, Cefotaxime, Ceftriaxone, and Cefuroxime and gave the same zone diameter range as adapted by CLSI [2014] document M100 S24 and nine considered potential ESBL producers
ABSTRACT
Enterococci is an emerging multidrug-resistant global opportunistic pathogen and is acquiring increasing importance as a nosocomial pathogen. This study aimed to determine the prevalence of nosocomial enterococci infections in the intensive care units of Assiut University Hospitals. This study included 285 patients who developed hospital acquired infections. They were admitted to different intensive care units [ICU] from March 2013 to March 2014. Appropriate clinical samples were collected from the patients after consent and were cultured for isolation and biochemical identification. A total of 30 strains of enterococci were isolated from 285 patients [10.5%]. The commonest clinical manifestation were lower respiratory tract infections [86.6%], wound infections [6. 7%] and urinary tract infections [6. 7%]. The chest ICU showed the highest percentage of isolation [66. 7%]. We conclude that nosocomial enterococci infections are significant in Assiut University Hospitals. High prevalence of enterococci in the lower respiratory tract infections
ABSTRACT
This study aimed to isolation of Acinetobacter spp, detect resistance patterns of isolated strains, phenotypic and genotypic detection of Metallo-beta-lactamase and typing of isolated strains from clinical and environmental respectively. this study includes 440 different clinical specimens, 672 environmental samples, inoculated on different culture media, confirmation of isolates by API20NE, PCR to detect 16SrRNA-23SrRNA gene, determine susceptibility pattern of isolates to different antibiotics and phenotypic and Genotypic detection of bla -OXA 51-like gene. 24 strains of Acinetobacter [5.45%] were isolated from 440 clinical samples, 27 strains of Acinetobacter were isolated from 672 environmental samples [4.017%]. Tetracycline was the most active drug against multi-drug resistant A.baumannii. [48/51 or 94%] of Acinetobacter isolates showed increase in zone of inhibition around IPM/EDTA disc compared with IPM disc alone. [49/51 or 96%] of Acinetobacter isolates were detected by presence of 16srRNA - 23 srRNA gene [universal gene present in all Acinetobacter species]. [49/51 or 96%] of isolated Acinetobacter spp showed band with blaOXA-51-like" genes. [37/51 or 72.5%] Acinetobacter isolates showed positive bands for class I integrase gene [gene responsible for multi drug resistance and outbreaks in hospitals]