ABSTRACT
The present meta-analysis aims to assess the evidence regarding the diagnostic accuracy and performance characteristics of the colorimetric redox indicator [CRI] assay with a special emphasis on the use of the resazurin microtiter assay [REMA] for determination of primary anti-tuberculosis drug resistance. By updating previous literature searches in Medline PubMed, ISI Web, Web of Science and Google academic databases of the REMA test for determination of primary anti-tuberculosis drug resistance, this meta-analysis includes 14 studies for isoniazid [INH]; 15 studies for rifampicin [RIF]; 6 studies for streptomycin [STR]; and 5 studies for ethambutol [EMB]. SROC curve analysis was performed for meta-analysis and diagnostic accuracy was summarized.: Pooled sensitivity was 96% [94-98%] for INH, 97% [95-98%] for RIF, 92% [87-96%] for EMB and 92% [88-95%] for STR. Pooled specificity for INH, RIF, EMB and STR was 96% [95-98%], 99% [98-99%], 86% [81-89%] and 90% [87-93%], respectively. Susceptibility testing results had been obtained in 8-9 days. In conclusion, REMA seems to be a reliable test for the determination of multidrug resistant [MDR] isolates in laboratories with limited resources. However, few studies for STR and EMB have been found, and costeffectiveness studies need to be determined to recommend its widespread use
ABSTRACT
Background: The concept of genetic factors playing a role in the pathogenesis of lung cancer has gained increased attention. The present study was undertaken to examine the question of HLA association with lung cancer and to investigate the effects of HLA on survival time
Methods: The distribution of HLA class I [A, B, C] antigens and class II [DR, DQ] alleles were studied in 81 unrelated Turkish patients with lung cancer. The HLA status of patients was compared with that of a control group consisting of 117 ethnically matched healthy donors. HLA class I antigens were studied by Terasaki?s microlymphocytotoxicity test and HLA class II alleles were studied by polymerase chain reaction with the sequence specific primer [PCR-SSP] low resolution method
Results: Only the frequencies of HLA-B51 and -DRB1x15 were lower in the lung cancer group compared with the healthy control patients. In a univariate analysis, age [P=0.03], Karnofsky Performance Status [P=0.0001], stage [P=0.01], HLAA24[9] [P=0.008], HLA B53 [P=0.0006], HLA B63[15] [P=0.01], HLA B64[14] [P=0.01], HLA B65[14] [P=0.01] and HLA CW5 [P=0.01] were significant prognostic factors. In a multivariate analysis, Karnofsky Performance Status [P=0.001], stage [P=0.02], HLA B53 [P=0.03] and HLA B64[14] [P=0.03] were independent prognostic variables
Conclusion: This study demonstrates different HLA types among patients with lung cancer and healthy control subjects. Our results suggest that HLA antigens might affect the prognosis in lung cancer. Further investigations are warranted to delineate any possible role of the HLA system in the pathogenesis and prognosis of lung cancer