ABSTRACT
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ABSTRACT
Persistent vesical dysfunction is often encountered after long term bladder overdistension caused by urinary retention. Experimentally, acute overdistension has also been implicated in the pathogenesis of the response to partial outlet obstruction. In the present study, we evaluated the expression of iNOS in the partially obstructed rat bladder and presupposed that NO is responsible for the prolonged micturition problem after partial outlet obstruction of the rat bladder. Female Sprague-Dawley rats weighing 150~200g were used. Individual bladders were obtained from unoperated control rats, and from rats at 6, 12, 48, 72 hours, 5 and 7 days after partial urethral obstruction. Total RNA was extracted from each of these tissue. The expression of mRNAs were assessed by Northern blot analysis. The band intensity of the autoradiographs measured by densitometry were compared between the obstructed and control group. The expression of mRNA for iNOS was detected in the control rats. The expression showed a sharp increase at 6 hours (342% increase) after obstruction and returned to normal by 24 hours. In this study, we show that iNOS increases in the rat bladder after operation and these results suggest that overproduction of NO may be a possible mechanism for the prolonged bladder dysfunction after partial bladder outlet obstruction.
Subject(s)
Animals , Female , Humans , Rats , Blotting, Northern , Densitometry , Nitric Oxide Synthase , Nitric Oxide Synthase Type II , Rats, Sprague-Dawley , RNA , RNA, Messenger , Urethral Obstruction , Urinary Bladder Neck Obstruction , Urinary Bladder , Urinary Retention , UrinationABSTRACT
During renal ischemia, ATP is degraded to hypoxanthine. When xanthine oxidase converts hypoxanthine to xanthine in the presence of molecular oxygen, superoxide radical (O2) is generated. This is toxic to cellular membranes through lipid peroxidation and may play an important role in the ischemic damage of the kidney. At the cellular level, with reperfusion there is accumulation of calcium and this potentiates oxygen free radical injury. The purpose of the present study is to determine whether oxygen free radicals and calcium play a role in mediating injury after renal ischemia. The ability of oxygen free radical scavenger (SOD) and calcium membrane blocker (verapamil) to protect renal function in the rabbit after renal ischemia was determined. The New-Zealand white rabbit was explored and occluded both renal arteries for 60 minutes with microvascular clamps. Group 1 (n=7) had normal saline infused into the both renal arteries followed by 60 minutes ischemia, group 2 (n=5) had SOD (10mg/kg) infused into the both renal arteries just before clamping, group 3 (n=5) had verapamil 15mg/kg infused. The results were as follows. Plasma creatinine in the group 2(1.7+/-0.1 mg/dl) was lower than group 1 (2.6+/-0.2 mg/dl) (p<0.05). Creatinine clearance and Ucr/Pcr in the group 2 (4.8+/-0.2ml/min, 19.8+/-2.6) was higher than group 1 (1.5+/-0.1 ml/min, 19.8+/-2.6) (p<0.05). Urine osmolarity in the group 2(574.6+/-22.3 m Osm/kg) was higher than group 1 (342.27+/-84.7 m Osm/kg). The function of solute handling was more reserved in the group 2 than group 1. (FeNa+ of group 1 vs. group 2 ; 6.9+/-0.6, vs. 2.0+/-0.3) (p<0.05). There was no difference between group 1 and 3 except Ccr. From these observations we conclude that free radical scavengers provide significant protection from the injury to the kidney and increased intracellular calcium potentiates renal injury during reperfusion.
Subject(s)
Adenosine Triphosphate , Calcium , Constriction , Creatinine , Free Radical Scavengers , Free Radicals , Hypoxanthine , Ischemia , Kidney , Lipid Peroxidation , Membranes , Negotiating , Osmolar Concentration , Oxygen , Plasma , Renal Artery , Reperfusion , Superoxides , Verapamil , Warm Ischemia , Xanthine , Xanthine OxidaseABSTRACT
It had heen emphasized the necessity for upper gastrointestinal endoscopic examinations in patients. Who has had gastrectomy and presents persisting gastrointestinal symptoms. This study was conducted to evaluate endoscopic findings and clinical symptoms in patients following gastrectomy. (continue...)