ABSTRACT
Objective To study the surgical techniques and clinical experience of the total laparoscopic splenectomy .Methods Retrospective analysis 31 cases were performed laparoscopic splenectomy from January 2003 to April 2015 .Among them ,there were 12 cases with liver cirrhosis and hypersplenism ,5 cases with spleen hemangiomatosis ,3 cases with splenic lymphoma ,3 cases with splenic cyst ,2 cases with obsolete rupture of spleen hematoma ,6 cases with idiopathic thrombocytopenia .During surgery ,Li‐gaSure was used to deal with stomach ligament splenic artery .Using silk or endoscopic linear cutters to process splenic pedicle indi‐vidually .Results All operations were performed successfully without conversion to open surgery .13 patients were cutting off splenic pedicle by ligating of splenic portal blood vessel and 18 patients were using endoscopic linear cutters .The operation time was 80-240 min ,mean (130 ± 35)min ,the blood loss was 60-500 mL ,mean (150 ± 80)mL ,hospital stay was 8 .3 days .There was no mortality and pancreatic leakage complications .Conclusion On the basis of open surgery ,evaluating carefully before operation ,se‐lecting appropriate cases ,using different laparoscopic instruments ,handling splenic pedicle individually ,can make the laparoscopic splenectomy be a more safe and useful operation modality .
ABSTRACT
OBJECTIVE: To observe the effects of astragalus polysaccharides(APS) combining with total ginsenoside (TG) on the proliferation of T cells in peripheral blood of healthy adults and on the immune function of mice after treatment with cyclophosphamide (CY) . METHODS: The proliferation rate of T cells in peripheral blood was detected by methyl thia-zolyl tetrazolium(MTT) colorimetric analysis; the weight of immune organs such as spleen and thymus etc. in mice were detected. RESULTS: The proliferation of T cells in human peripheral blood could be enhanced if the concentration of APS remained within 25-100?g/ml and that of TG remained within 5-20?g/ml; the proliferation rates were 102.3%-141.1% and 113.9%-163.1% , respectively; the figure stood at 262.3%-383.0% when APS and TG used in combination. The spleen indexes of APS group and the APS plus TG group increased significantly vs. the CY group(P