ABSTRACT
<p><b>OBJECTIVE</b>To understand the neutralized antibody level of the poliomyelitis among healthy people and provide scientific evidence for the immunization strategy since routine and intensified immunization with oral polio vaccine (OPV) in Fujian province.</p><p><b>METHODS</b>The poliomyelitis antibody level of healthy people were detected by neutralization test of the micro cells.</p><p><b>RESULTS</b>The neutralizing antibody positive rates were 99.0%, 99.3%, 97.5% and GMTs were 1:79.1, 1:31.2, 1:24.7 for polio I, II, III respectively in 400 serum specimens from 1-59 years old. GMTs present a trend of decreasing as age's increasing.</p><p><b>CONCLUSION</b>A protective barrier had been built against poliomyelitis in healthy people in Fujian province through routine and intensified immunization with OPV.</p>
Subject(s)
Adolescent , Adult , Child , Child, Preschool , Female , Humans , Infant , Male , Middle Aged , Young Adult , Antibodies, Neutralizing , Blood , Allergy and Immunology , Antibodies, Viral , Blood , Allergy and Immunology , China , Health Status , Poliomyelitis , Allergy and Immunology , Virology , Poliovirus Vaccine, Oral , Allergy and Immunology , Population Surveillance , VaccinationABSTRACT
<p><b>OBJECTIVE</b>To ascertain the pathogen of aseptic encephalitis epidemic in Long-Yan city in Fujian, and to find out the genetic characteristics of the virus.</p><p><b>METHODS</b>Rapid detection of enteroviral RNA by reverse transcription polymerasechain reaction (RT-PCR) was directly carried out in cerebrospinal fluid(CSF) to isolate and identify the viruses from CSF at the same time, and to detect the neutralization antibody in two serum specimens collected in acute and convalescence phase. Nucleotides of VP1 region was also analyzed by constructing phylogenetic tree.</p><p><b>RESULTS</b>ECHO 19 infection was rapidly diagnosed and sequence analysed by RT-PCR, and then echovirus type 19 from 16 of 30 CSF samples (53.33%) was isolated and detected using RD and Hep-2 cells simultaneity. The titer of ECHO 19 neutralization antibody became positive or increased by 4 times from acute to convalescence phase in 4 of the 5 patients. Phylogenetic analyses of the VP1 genes of these isolates showed that their nucleotides identity were 98.9% -100.0% which were different from those ECHO 19 from GeneBank database by 13.0%-22.4%.</p><p><b>CONCLUSION</b>The etiology of the epidemic of aseptic encephalitis was attributed to ECHO 19. The method of molecular identification not only provided rapid diagnosis of enterovirus infections, but also information about the genetic character of the viruses.</p>
Subject(s)
Humans , Antibodies, Neutralizing , China , Epidemiology , Echovirus Infections , Diagnosis , Allergy and Immunology , Virology , Encephalitis, Viral , Epidemiology , Virology , Enterovirus B, Human , Genetics , Phylogeny , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
0.05).(3)The levels of CRH and DHEA-S in umbilical cord blood of PL(7.8?3.3)ng/L,and(514?295)?g/L,respectively and of TL (7.7?4.1)ng/L,and(483?207)?g/L,were higher than that in term not in labor(4.8?2.4)ng/L, and(360?80)?g/L,respectively(P 0.05).In PL,the level of CRH in umbilical cord blood and the expression of CRH mRNA in placentas and fetal membranes were correlated with each other(r=0.935 and 0.853,P