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Objective:To establish reverse-phase high-performance liquid chromatography and simultaneously determine gallic acid, methyl gallate, corilagin, Sennoside B, and Sennoside A levels in Sana preparations.Methods:From January to December 2021, Phenomenex Hydro-RP 80A column (4.60 mm × 250 mm, 4 μm) was used. Elution was conducted using mobile phases methanol (A)-0.2% formic acid (B). The following gradients were applied: 1%-3%A for 0-18 minutes, 3%-15%A for 18-19 minutes, 15%-17%A for 19-40 minutes, 17%-25%A for 40-45 minutes, 25%-35%A for 45-65 minutes, 35%-60%A for 65-95 minutes, 60%-90%A for 95-96 minutes, 90%-1%A for 96-97 minutes. The flow rate was 1.0 mL/minute. The column temperature was 35 ℃. The detection wavelength was 270 nm.Results:The linear ranges of gallic acid, methyl gallate, corilagin, Sennoside B and Sennoside A were 0.182-1.099 μg ( r = 0.999 9), 0.046-0.278 μg ( r = 0.999 2), 0.266-1.598 μg ( r = 0.999 4), 0.172-1.036 μg ( r = 0.999 2), and 0.176-1.056 μg ( r = 0.999 9). The average dosing recovery rates were 100.02%, 99.14%, 99.38%, 101.77%, and 100.92%, respectively. Conclusion:Reverse-phase high-performance liquid chromatography can be used for quality control of Sana preparations because of high accuracy, sensitivity, reliability, and reproduction.
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Objective To investigate the influence of liraglutide combined with metformin and simple metformin on insulin re‐sistance in the patients with type 2 diabetes mellitus(T2DM ) .Methods Thirty patients with T2DM treated by liraglutide combined with metformin were collected as the experimental group ,while other 30 patients with T2DM treated by single metformin were col‐lected as the control group .The changes of HbA1c ,HOMA‐IR ,adiponectin ,NO and ET before treatment and at 3 months after treatment were compared between the two groups .Results The decrease degree of HbA1c ,HOMA‐IR ,NO and ET and the in‐crease degree of adipodectin in the experimental group were more significant than those in the control group with statistical differ‐ences between the two groups(P<0 .05) .Conclusion Liraglutide has more significant effect than metformin for improving the in‐sulin resistance in the patients with T2DM .
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Objective To analyze the correlation between body mass index(BMI)and percentage body fat(PBF)in people of dif-ferent nationality and gender,and assess the value of BMI and PBF in the diagnosis of obesity.Methods A total of 925 healthy a-dults who underwent physical examination in the hospital were enrolled in the study,bioelectrical impedance measurement was used to measure the weight,PBF,then analyze the correlation between BMI and PBF in people of different nationality and gender.Results In the normal and overweight population classified by BMI,;In underweight,overweight and obesity people,PBF of Han women were higher than that of Uighur women(P <0.05 ).In people of different gender and ethnic group,PBF was positively correlated with BMI(P <0.05).Using BMI≥28 kg/m2 as gold standard for the diagnosis of obesity,PBF have good sensitivity and low speci-ficity for the diagnosis of obesity in both of the two ethnic groups.Conclusion Compared with Uygur,Han have higher PBF at the same level of BMI.There is a malconformation between BMI and PBF.Evaluation of obesity could not simply rely on BMI,and should be analyzed combined with PBF.
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Objective To investigate the relation between the characteristics of body fat distribution and the cardiovascular risk factors in some patients with type 2 diabetes mellitus (T2DM)in Xinjiang region.Methods The percentage of body fat (PBF)and visceral fat area(VA)were determined in 155 cases of T2DM by using the Inbody 720 body composition analyzer.At the same time triglycerides(TG),total cholesterol(TC),high density lipoprotein(HDL-C),low density lipoprotein(LDL-C),blood pressure(BP), glycosylated hemoglobin HbA1c,body weight(W),body height,waist circumference ratio(WC)and hip circumference(HC)were also determined.The body mass index(BMI)and the waist to hip ratio was calculated.Results In both men and women,BMI in the subjects with visceral fat obesity(VFO)was higher than that with subcutaneous fat obesity(SFO).In males,TG in VFO was signif-icantly higher than that in SFO.PBF,TC and LDL-C in the female T2DM patients were significantly higher than those in the male T2DM patients.VA and PBF in the minority male patients were significantly higher than those in the Han nationality male patients (P <0.05).After correcting the age,course of disease and BMI,WHR in the female patients was positively correlated with DBP and PBF was positively correlated with LDL-C.In the male patients,there were correlations between BMI with SBP,between VA with DBP,and between VA with PBF and HDL-C;in the female patients,there were correlations between BMI with SBP and be-tween WHR with DBP.Conclusion The body fat distribution in the patients with T2DM in Xinjiang region is dominated by VFO. The cardiovascular risk factors are not only associated with the increase of body fat content,but more closely associated with VFO, moreover the differences in genders and nationalities exist.
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Sleep disorders are common diseases in children,although in recent years,pediatric sleep medicine had sustained development,but sleep problems in children's daily life are still more common and serious,the visiting rate is becoming higher.Pediatric sleep disorder can result in damage to the cognitive growth and behavior of children.In order to capture the majority of pediatric medical workers and parents attention,this review focuses on current diagnosis and therapy for selected common pediatric sleep disorders including obstructive sleep apnea syndrome,restless legs syndrome and narcolepsy.
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Objective To assess the values of growth differentiation factor-15 (GDF-15) and N-terminal probrain natriuretic peptide (NT-proBNP) for the diagnosis of children with congenital heart disease (CHD) combined with heart failure.Methods Among the children under treatment at the First Affiliated Hospital of Xinjiang Medical University from Jan.2011 to Dec.2012,206 children those suffered with CHD were selected as the case group.One hundred and five children without CHD or heart failure were selected as the control group.Improved Ross score ≥ 3 grades was taken as reference standard for heart failure.The case group was divided into non-heart failure group (0-2 grades,112 cases) and the heart failure group (3-12 grades,94 cases).The heart failure group was divided into mild heart failure group (3-6 grades,38 cases),moderate heart failure group (7-9 grades,32 cases) and severe heart failure group (10-12 grades,24 cases).Levels of plasma GDF-15 and NT-proBNP were tested.In the meanwhile,the left ventricular ejection fraction (LVEF) was tested with ultrasonic cardiogram.Linear correlation analysis was performed for evaluating levels of plasma GDF-15 and NT-proBNP against the cardiac function grading in Ross scores and the LVEF,respectively.ROC curve was made to determine critical point or threshold value of diagnosis and assess values of levels of plasma GDF-15 and NT-proBNP for diagnosis of congenital heart disease combined with heart failure.Results Comparison of age and gender differences between the case group and the control group showed no statistically significant(x2 =4.233,4.360,P ＞ 0.05).Levels of plasma LogGDF-15 and LogNT-proBNP in the heart failure group were significantly higher than those values of the non-heart failure group and the control group(F =115.2,63.2,all P ＜ 0.01).Levels of plasma GDF-15 and NT-proBNP increased with the severity of heart failure and were positively correlated with improved Ross scores (r =0.890,P ＜ 0.01) and negatively correlated with LVEF (r =-0.652,P ＜ 0.01).GDF-15 was considered in combination with NT-proBNP,the specificity(80.2％) and precision(77.9％) for diagnosis of heart failure could be enhanced when plasma GDF-15 ＞ 831.6 ng/L coexisted with NT-proBNP ＞ 759.8 ng/L.Conclusions Plasma GDF-15 and NT-proBNP can help the diagnosis of heart failure caused by CHD in children.The increase of these two factors is closely related to the severity of clinical heart failure.Combination of these two factors for test can enhance specificity and precision for diagnosis of heart failure.
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OBJECTIVE@#To observe the effects of xipayi mouth rinse of different concentrations on the activities of interleukin-6 (IL-6) from human gingival fibroblast (HGF) induced by lipopolysaccharide (LPS).@*METHODS@#HGF was stimulated with LPS at 25 g/mL, and the radioimmunoassay (RIA) was used to examine the effect of xipayi mouth rinse at 12.5 approximately 200 g/mL on the secretion of IL-6 in the supernatant of the cell culture.@*RESULTS@#IL-6 secreted by human gingival fibroblast was significantly inhibited by xipayi mouth rinse in a dose dependent manner.@*CONCLUSION@#Xipayi mouth rinse can inhibit the secretion of IL-6 from HGF induced by LPS, suggesting the anti-inflammatory effect of xipayi mouth rinse to treat and prevent periodontal diseases.
Subject(s)
Humans , Anti-Inflammatory Agents , Pharmacology , Cells, Cultured , Depression, Chemical , Drugs, Chinese Herbal , Pharmacology , Fibroblasts , Metabolism , Gingiva , Cell Biology , Interleukin-6 , Metabolism , Lipopolysaccharides , MouthwashesABSTRACT
OBJECTIVE@#To observe the effects of xipayi mouth rinse on the DNA synthesis and change of cell cycles of human gingival fibroblast (HGF) induced by lipopolysaccharide(LPS).@*METHODS@#HGF was stimulated with LPS at 25 mg/L. Flow cytometry was used to examine the effect of xipayi mouth rinse at 25 mg/L on the DNA synthesis and change of HGF cell cycles.@*RESULTS@#The percentage of HGF in G( 1) phase increased after the cells were induced by LPS, while the percentage of HGF in S phase decreased. Xipayi mouth rinse could ameliorate this phenomenon.@*CONCLUSION@#Xipayi mouth rinse can significantly ameliorate the inhibitory effect of LPS on the proliferation of HGF, suggesting the anti-inflammatory effect of xipayi mouth rinse in the treatment and prevention of periodontal diseases.