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BACKGROUND:Placental mesenchymal stem cells are rich in source and easily obtained, which can differentiate into osteoblasts, nerve cells and liver cells. Additionally, there is no immune rejection and ethical issues in the clinical application. Therefore, placental mesenchymal stem cells are considered to be a good source of adult stem cells.OBJECTIVE:To observe the effect of placental mesenchymal stem cell transplantation in the repair of endometrial lesions in rats.METHODS:Endometrial damage models were established in rats by means of thermal damage, and on the 15th day after modeling, these rat models were randomly divided into four groups (n=10 per group):intrauterine injection of 1 mL of allogeneic placenta mesenchymal stem cell suspension (intrauterine transplantation group), intrauterine injection of the same amount of PBS (intrauterine control group), tail vein injection of 1 mL of allogeneic placenta mesenchymal stem cell suspension (intravenous transplantation group), and tail vein injection of the same amount of PBS (intravenous control group). The female rats experiencing the third estrus after modeling were caged with male rats to observe whether the vaginal plug appeared. The female rats were killed the same day when the vaginal plug was observed, and uterus tissues were taken to detect the number of endometrial glands as well as perform immunohistochemistry and western blot detection.RESULTS AND CONCLUSION:The number of endometrial glands was highest in the intrauterine transplantation group followed by the intravenous transplantation group, and lowest in the two control groups (P < 0.05). The expression of integrin αvβ3 shown by immunohistochemistry and western blot was highest in the intrauterine transplantation group followed by the intravenous transplantation, and lowest in the two control groups (P < 0.05). These findings indicate that the placental mesenchymal stem cell transplantation can repair damaged endometrial tissues in rats to different degrees,by increasing endometrial glands count and improving the endometrial receptivity.
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BACKGROUND:Placental mesenchymal stem cels are becoming a new source of seed cels because of wide range of sources, low immunogenicity and not involving ethical issues. OBJECTIVE:To elaborate the sources, biological characteristics and latest application of placental mesenchymal stem cels. METHODS:Literature search was performed in PubMed, ScienceDirect, OvidSP, CNKI databases for relevant literatures published from 2003 to 2015. The key words were “placenta, mesenchymal stem cels, placenta mesenchymal stem cels, cel transplantation, application mechanism” in Chinese and English, respectively. Then, 57 papers were further analyzed and reviewed in line with the theme. RESULTS AND CONCLUSION:Placental mesenchymal stem cels have been isolated and cultured successfuly, and confirmed to have multi-differentiation potential. A large number of placental mesenchymal stem cels have been used in the experimental animal and clinical researches, and they have a great potential in bone tissue engineering, revascularizaion and nerve repair. However, the specific mechanism underlying the application of placental mesenchymal stem cels is not clear. In order to ensure the safety and effectiveness, there are stil many problems to be further studied before placental mesenchymal stem cels are widely used in clinic.
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A cross-sectional study in 116 patients with polycystic ovary syndrome (PCOS) was carried out by using the short-form-36 health survey (SF-36), serf-rating anxiety scale ( SAS), and self-rating depression scale (SDS) from January 2008 to April 2008 in our reproduction center. In comparison with the healthy controls, the PCOS patients showed decreased quality of life (QOL) for all items of SF-36 (P<0.05) except for body pain or role-physical scales. The differences in SAS and SDS between the PCOS and the healthy controls were statistically significant (P<0.05). Our study results confirm the negative impacts of PCOS over the quality of life;subfertility, obesity, hirsutism, acne, complications, menstrual irregularity and educational levels might contribute to the reduced QOL.
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Objective To sequence follicle stimulating hormone receptro (FSHR) promoter of the ovarian granulocyte and initially research the molecular mechanism of the poor ovarian response. Methods To study the relationship between FSHR promoter mutation of ovarian granulocyte and ovarian respone. The 263 bp DNA fragments before FSHR 5'initiation site in 70 cases of patients with poor ovarian respone and 88 cases of patients with ovarian normal respone who were in the cycle of IVF-ET were sequenced, Results There were 63 cases which occurred 29th site G → A point mutation in 158 women and the mutation rate was 40. 0%. Mutation rate [ 60. 0% ( 42/70 ) ] of 29th site G → A in group of poor ovarian respone was significantly higher(χ2 = 21. 450,P < 0. 01 ) than normal response group [ 23.9% ( 21/88 ) ]. There was no obviously variability ( t = 0. 457, P 0. 05 ) of basic FSH values between two groups [ G/G group was (7.2 ± 2. 3) U/L, G/A & A/A group was (7. 1±2. 0) U/L];there was obviously variability (t = 35. 81 ,P < 0. 05 ) in the number of follicles sinus between two groups ( G/G group was 14. 2±1.3, G/A & A/A group was 4. 5±0. 8 ) ;there was obviously variability ( t = 40. 35, P < 0. 05 ) in the number of ovum pick-up between two groups ( G/G group was 14. 0±1.2, G/A & A/A group was 4. 5±1.1 ) ;there was obviously variability (t =25. 80,P <0.05) of FE2-peak value between two groups [G/G group was (2 865±557) pmol/L, G/A & A/A group was (880±211 ) pmol/L] ;there was obviously variability (t =40. 22 ,P <0. 05) in the number of mature eggs ( G/G group was 13.6±1.2, G/A&A/Agroupwas4.3±0. 9).Conclusion The 29th site of FSHR promoter significantly affect the activity of FSHR promoter. Mutation of G→A can weaken promoter activity, so that ovarian granulocyte poor respone to FSH.
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This study was to investigate the role of urethral catheter balloon dilatation(F16)in the treatment of intrauterine adhesion after hysteroscopic surgery.A total of 523 patients with severe intrauterine adhesion(IUA)following hysteroscopic surgery underwent urethral catheter balloon dilatation during April 2000 and December 2005.During 3～15 months'follow-up,normal menses were seen in 340 patients (65.0％),nearly normal menses in 165 patients(31.5％),and menoschesis in 18 patients(3.4％).302 patients showed normal uterine cavity with no recurrent endometrial adhesion,203 patients(38.8％)were found slight IUA,and only 18 patients(3.4％)still presented with severe adhesion.No operative complication was reposed.and the pregnant rate was 47.6％.In summary.urethral catheter balloon dilatation.based therapeutic strategies is safe.effective and beneficial.
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Four hundred and five pregnant women whose husbands had positive HBeAg,HBsAg and anti HBc were recruited in the study.Among them.218 women with positive HBsAb were completely randomized into group A and group B,while 187 cases with negative HBsAb formed group C.Women in group A and group C were injected with hepatitis B immunoglobulin(HBIG)before delivery.All newborn babies in 3 groups received routine HCV vaccination.in addition babies in group A and group C also received HBIG iniection within 24 h after birth.The HBV infeetion rate of newborns was 12.1%in group A,12.8%in group B and 23.0%in group C respectively.
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BACKGROUND: Skeletal muscle-derived stem cells (MDSCs), another adult pluripotent stem cells, have become a hot topic in the field of gene therapy and cell-based tissue engineering. It has wide prospect in treating stress incontinence by periurethral injection of patients' MDSCs.OBJECTIVE: To investigate the method of culturing MDSCs in vitro so as to provide experimental basis for treating stress incotinence by injecting MDSCs.DESIGN: Repeated observation.SETTING: Department of Reproductive Medical Center and Department of Urology, Renmin Hospital of Wuhan University.MATERIALS: This experiment was conducted at the Laboratory of Department of Urology , Renmin Hospital , wuhan University from December 2003 to May 2004. Totally 20 female SD rats , aged 4 to 6 weeks , were involved . Type- Ⅺ pancreatin, collgenase , pancreatin ,polylysine (Sigma), dispase enzyme (Gibco) , chick embryo extract (self made).METHODS: ① Rats were anesthetized intraperitoneally with 10 g/Lpentobarbital sodium (30 g/kg). Under aspetic condition, gastroeneminus were isolated and immediately put into pre-cooled DMEM media (Gibco)containing antibiotics and then removed into the hood. After washed with D-hank's solution three times, muscle biopsies were removed of fascia,tendon, nerve and blood vessels and minced into small pieces about 1-3 mm3,and then transferred into a centrifugation tube. 0.2% collgenase-type Ⅺ(Sigma) and 0.1% pancreatin were added to the left tissue . Skeletal muscle cells of the rats were isolated with collagenase. ② Differential attachment was used to purify the skeletal muscle cells of the rats. After screened with cell screen cloth , cell suspension was transferred into a polylysine-coated flask and cultured at 37 ℃ in a humid atmosphere with 0.05 CO2 in air for 1 hour. All the cells that did not adhere to the flask were then transferred to another flask with new culture medium and cultured for approximately 1 hour at 37 ℃ .Again,the non-adhering cells were transferred to another flask and were incubated at 37 ℃ overnight.The technique was carried out for an additional 4-5 days. This operation was repeated every 24 hours until the fifth day. Those cells attached on days 5-6 were MDSCs.③ After they grew with 70% confluence , the cells were digested with trypsin and passaged at the ratio of 1:2. After digestion, the generative cells were inoculated to the culture plate with 6-well cover glass. Growing culture medium was added and cell slide was prepaared 24 hours later. The expression of specific protein antigen and stem cell antigen-1 of the cultured cells were identified with immunohistochemical staining.MAIN OUTCOME MEASURES: Morphology and identification of MDSCs.RESULTS: ① Morphology of MDSCs: Cells isolated from skeletal muscle tissue took the form of spheres and had high refraction. When subcultured,they began to adhere at the 12th hour, at that time they were still round,and complete their attachment at the 48th hour. Then they began to expand and ultimately became fusiform-shaped or spindle-shaped, having two poles and small size. As time going, they fused with each other to form mature poly-nuclear myotubes. ② Identification results of muscle-derived stem cells: MDSCs were desmin and stem cell antigen-1 (Sca-1) positive specific for some stem cells. Red fluorescence effluenced from cytoplasm was found under the fluorescence microscope and the positive rate reached 90%.CONCLUSION: MDSC belongs to adult stem cells and has the advantages of rich source, low immunogenicity and long survival after transplantation. High-purity muscle-derived stem cells can be obtained through primary culture , and immunohistochemical technique can identify muscle-derived and specific characteristics of stem cells, so it has a broad application perspective in tissue engineering and gene therapy.
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Objective To evaluate the clinical value of hysteroscopy before in vitro fertilization and embryo transfer(IVF-ET).Methods From December 2003 to May 2005,130 patients received hysteroscopy in our hospital because of IVF-ET failure.Their intrauterine diseases were treated by hysteroscopy,and the abnormal tissues were examined pathologically.Results Among the 130 cases,87 had intrauterine diseases(87/130,66.9%) including endometritis in 40(40/130,30.8%),endometrial polyps in 23(23/130,17.7%),intrauterine adhesion in 20(20/130,15.4%),and submucous leiomyoma in 4(4/130,3.1%).In these patients,67 underwent IVF/ICSI or frozen embryo transfer within one year after the hysteroscopy,of which 20 patients achieved pregnancy.The clinical pregnancy rate was about 30%.The other 20 cases did not repeat IVF-ET in 10-20 months after the hysteroscopy,and had no pregnancy without contraception.Conclusion Pre-transfer hysteroscopy is helpful to avoid IVF-ET failure owing to intrauterine diseases.