ABSTRACT
Objective To set up a simple and accurate method for the determination of total Glucosides in Paeonia lactifloria (TGP) by HPLC.Methods Benzoic acid being as contiol,the amounts of TGP were obtained from the following formula :TGP( ) = benzoic acid() ?480. 27/ 121. 121. Benzoic acid was determined by HPLC. Chromatographic conditions concluded YMC-Pack ODS-A(5?m,150?4.6mm)column and the mobile phase consisting a mixture of MeOH - 0.05mol?L - 1KH2PO4 - HAC - isopropanol (85:155:4:6). Benzoic acid was detected at 230nm wavelength.Results The calibration curves of Benzoic acid were in good linearity over the range of 0.1~2g(r=1.0000). The average recovery of Benzoic acid was 101.12(n=9,RSD=2.49). Conclusion This method is simple and can be easily repeated.