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Objective To evaluate effects of artesunate on rosacea-like inflammation in mouse models.Methods Twenty-five male BALB/c mice aged 7 weeks were injected subcutaneously with 40 μ1 antibacterial peptide LL-37 into the back once every 12 hours for 4 sessions to establish mouse models with rosacea-like inflammation.These 25 mice were randomly and equally divided into 5 groups:after each injection of LL-37,model group were gavaged with sodium chloride physiological solution,treatment groups gavaged with 25,50 and 100 mg/kg artesunate solution separately,and positive control group gavaged with 30 mg/kg doxycycline hydrochloride solution.Another 5 healthy mice injected subcutaneously with pure water into the back for 4 sessions served as blank control group.Forty-eight hours after the initial injection of LL-37,changes in skin lesions and the intensity of erythema were assessed.Skin tissues at the dorsal injection site were resected and subjected to HE staining,the tissue structure was observed and the number of inflammatory cells was counted.Enzyme-linked immunosorbent assay (ELISA) was performed to estimate the activity of myeloperoxidase (MPO) in skin lesions.Results The model group showed obvious inflammatory reactions,and significantly increased erythema score (3.20 ± 0.84),inflammatory cell count (517.27 ± 99.43) and MPO activity (0.57 ± 0.08) compared with the blank control group (all P ＜ 0.01).The positive control group showed significantly decreased erythema score (1.60 ± 0.89),inflammatory cell count (270.93 ± 124.63) and MPO activity (0.40 ± 0.05) compared with the model group (P ＜ 0.05,0.01,0.01,respectively).Moreover,the erythema score,inflammatory cell counts and MPO activity were all significantly lower in 50-(1.80 ± 0.84,286.00 ± 33.72,0.43 ± 0.05,respectively) and 100-mg/kg artesunate groups (1.40 ± 0.55,258.00 ± 36.44,0.40 ± 0.06,respectively) than in the model group (P ＜ 0.05 or 0.01).However,there were no significant differences in the erythema score,inflammatory cell count and MPO activity between 50-or 100-mg/kg artesunate group and the positive control group (P ＞ 0.05).Conclusion Artesunate can inhibit rosacea-like inflammatory reactions in mouse models,especially the middle-and high-dose artesunate.
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The first family doctor studio was established at 2011 in Shanghai,afterwards a wide variety of family doctor studios were set up over the country.Family doctor studio has been become a significant form and carrier to promote the family doctor service.However,there are still lacks of unified standards and perfect mechanisms for its operation and evaluation.To adapt the reform of community health service,it is urgent to standardize the family doctor studio,to promote the development of the community health service and the reform of the family doctor system.Based on Zhoujiaqiao mode,Changning District has kept exploring the operation mechanism,standardizing the management and service mode,and promoting brand building of the family doctor studio since 2012.The service mode and operation mechanism of family doctor studio with Changning characteristics has gradually formed.This article summarizes the construction,development and improvement of the family doctor studio.
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OBJECTIVE@#The aim of this study is to investigate the incidence and natural history of secretory otitis media(SOM) and hearing loss in newborns and infants with cleft palate, consequently, define its audiological criteria and to predict SOM early.@*METHOD@#Seventy-three newborns and infants with a cleft palate (146 ears) were monthly estimated by tympanogram, static compliance, acoustic stapedius reflex and auditory brainstem response (ABR) under natural sleep within one year of age.@*RESULT@#Au the infants with cleft palate had the suspected SOM in the first 6 months of life. Among children with cleft palate, the suspected SOM were most prevalent in the 3-month-age. 78. 8% infants with cleft palate had the confirmed SOM in the first 12 months of life. SOM were most prevalent in the 6-month-age. The SOM prodromal period was averagely 3. 8 months from suspected SOM to confirmed SOM. 56. 2% infants with cleft palate had a conductive hearing loss in the first 12 months of life. The conduction hearing thresholds of ABR (2-4 Hz) were averagely 48. 6 dBnHL.@*CONCLUSION@#The highest incidence of SOM and hearing loss in children with cleft palate appear in infants in the first 1 year of life. The process of SOM and hearing loss onset is progressive process. The infants with cleft palate should be estimated by ABR and acoustic immittance audiometry in each period of 2 or 3 months after birth.
Subject(s)
Female , Humans , Infant , Infant, Newborn , Male , Cleft Palate , Hearing Loss , Epidemiology , Incidence , Otitis Media with Effusion , EpidemiologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the inherent characteristics of Vibrio cholerae (V. cholerae) and other vibrios and their relationship.</p><p><b>METHODS</b>Polymerase chain reaction (PCR), DNA sequence analysis, randomly amplified polymorphic DNA (RAPD) analysis and average linkage cluster analysis were used to study 3 isolates of V. cholerae strains O139, three isolates O1 biotype El Tor, four isolates O1 biotype classical and 3 other vibrios.</p><p><b>RESULTS</b>V. cholerae O139 contained the genomic sequences of ctx A2-B as well as V. cholerae O1. V. cholerae and others vibrios were divided into 4 groups by fingerprint patterns of RAPD, that is (1) V. cholerae O139 and V. cholerae O1 El Tor; (2) V. cholerae O1 classical; (3) V. paraheamolyticus and V. vulnificus and (4) V. flluvialis. V. cholerae O139 DNA fingerprint of RAPD was consistent with the El Tor biotype: average linkage cluster distance was 0, and slightly different from the classical biotype, with a distance of 2.07. It was much more different from vibrio paraheamolyticus and others, with a distance of 6.76 - 8.54.</p><p><b>CONCLUSION</b>V. cholerae and other vibrios are polymorphic in inherent characteristics. The inherent characteristics of V. cholerae O139 are the same as El Tor biotype. O139 may have evolved from the El Tor biotype. The inherent characteristics of vibrio paraheamolyticus are the same as vibrio vulnificus.</p>