ABSTRACT
OBJECTIVE@#To determine the effect of exogenous hydrogen sulfide on experimental hepatic fibrosis in rats and its mechanism.@*METHODS@#Wistar male rats were randomly divided into three groups: a normal control group (n=8), a model group (n=8), and a hydrogen sulfide prevention group (n=8). The rat model of hepatic fibrosis was reduced by intraperitoneal injection of carbon tetrachloride (CCl4). The prevention group, in addition to intraperitoneal injection of 40% CCl4, was intraperitoneally administered H2S once a day until 8th week. After the experiment, the liver function and liver fibrosis were assayed. Liver tissue samples were used for histopathological changes. The expression of TGF-β1 in liver tissue was detected by RT-PCR and Western blot.@*RESULTS@#Compared with the model group, the levels of ALT, AST, HA, LN, and PC III in the sulfide group were significantly reduced (P<0.01 or P<0.05), ALB content was increased (P<0.05) in the serum, TGF-β1 expression was obviously reduced, and the degree of liver fibrosis was improved (P<0.05).@*CONCLUSION@#Exogenous hydrogen sulfide can effectively inhibit the development of hepatic fibrosis, reduce the expression of TGF-β1, and decrease the the sediment of extracellular matrix in the liver tissues.
Subject(s)
Animals , Male , Rats , Carbon Tetrachloride , Extracellular Matrix , Metabolism , Hydrogen Sulfide , Pharmacology , Therapeutic Uses , Liver , Pathology , Liver Cirrhosis , Pathology , Rats, Wistar , Transforming Growth Factor beta1 , MetabolismABSTRACT
0.05),and the antifebric time of group A was shorter than that of group B.No significant difference was found in the incidence rate of side reaction between two groups. CONCLUSIONS Ceftazidime and vancomycin combination is more effective than ceftazidime alone.