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Indian J Exp Biol ; 2002 Jan; 40(1): 109-10
Article in English | IMSEAR | ID: sea-56609

ABSTRACT

A gene encoding beta toxin was amplified by polymerase chain reaction from C. perfringens type C isolate and cloned in pUC 19 vector. The nucleotide sequence was identical with C. perfringens type B beta toxin gene sequence. The Southern hybridization using labelled beta toxin gene probe revealed the presence of positive signals only in beta producing C. perfingens.


Subject(s)
Animals , Bacterial Toxins/genetics , Blotting, Southern , Cloning, Molecular , Clostridium perfringens/classification , DNA Primers/chemistry , DNA, Bacterial/analysis , Genes, Bacterial , Molecular Sequence Data , Polymerase Chain Reaction , Restriction Mapping , Sequence Analysis, DNA
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