Assessment of hepatic regeneration after partial hepatectomy in albino rats with induced bone barrow suppression

The liver has a remarkable capacity to regenerate after injury. Within a week after partial hepatectomy [PH] hepatic mass is back essentially to what it was prior to surgery. Hematopoietic stem cells [HSCs] may contribute in the regeneration and the renewal capacity of hepatocytes. To investigate the origin of hepatocytes in liver regeneration whether from resident hepatic stem cells or from circulated HSCs after suppression of HSCs from bone marrow by Benzene This experimental study included 24 adult male albino rats. The studied animals were divided into 4 groups; first group [control group] was included 6 normal rats. No intervention was carried out to this group of rates. Second group [PH] group] was included 6 rats in which 70% PH was done. Third group [Benzene group] was included 6 rats of in which bone marrow [BM] suppression was carried out by Benzene injection. Ten injections were performed subcutaneously in a period of 3 weeks for induction of BM suppression. Fourth group [Benzene+ PH group] was included 6 rats in which BM suppression was carried out as 3rd group. Seventy percent PH was then done to them. Cytological changes during regeneration were assessed in all groups by; the number of binucleate cells and the restored number of hepatocytes. Mitotic index was performed in the second and the fourth groups. The percent of regeneration was also calculated. Immunohistochemistry technique was used for detection of CD34+ cells markers in liver tissues by using anti CD34+ cells antibodies [this technique is done in group 4 only]. Our results found that mean weights of rats and assumed liver weights, there were no statistical significant differences between the studied groups. PH group had shown higher regeneration rate than Benzene+ PH group; the former showed a mean loss of 19% [81 +/- 2.1% of regeneration] of their original weight by the end of the first week, and the latter a mean loss of 30% [70 +/- 0.7% of regeneration]. Number of binucleate cells there was a significant difference in benzene treated group compared to control group [p<0.05]. Mitotic index there was significant higher mitotic index in partially hepatectomized after bone marrow suppression by benzene than partially hepatectomized group [p<0.05]. We had used CD34 [CBRE8] antibody in our. It is indicated that the hepatocytes can regenerate the liver and restore its original size. Hematopoietic stem cells may habitat the endothelial cells in the liver. Their role in hepatic regeneration doesn't appear early after partial hepatectomy it mobilized from bone marrow to the damaged liver rlay in the liver tissues for long time [> 7 days] then differentiate into non parynchematous liver tissues

Role of tumor necrosis factor-alpha on hepatic regeneration after partial hepatectomy in adult male albino rats

This study aimed to evaluate the effect of TNF- alpha on liver regeneration after seventy percent partial hepatectomy of male rats. Rats were divided into three groups: control, untreated partially hepatectomised group and Pentoxifylline [a drug which blocks TNF-alpha] treated hepatectomised group. Each group had thirteen rats. Rats were killed on the 1[st], 3[rd], 5[th] and 7[th] days posthepatectomy. The removed part of the liver was weighed to detect the increase of the total liver weight on the previous days. A sample of the liver was taken for histological and immunohistochemical studies. The weight of liver in the untreated group showed gradual increase up to more than ninety nine per cent of the original liver weight on the7[th] day posthepatectomy. In the treated group, it reached only fifty five per cent of the original liver weight on the 7[th] day posthepatectomy. Mitotic activity in the untreated group was sharply increased on the 1[st] day then gradually decreased and disappeared on the 7[th] day. Treated group showed a decreasing in mitotic activity from the1[st] day and disappearing on the 3[rd] day. Immunohistochemical studies in untreated group showed intense cytoplasmic overexpression for TNF- alpha on the 1[st] and the 3[rd] days, moderate overexpression on the 5[th] day and mild overexpression on the 7[th] day. Treated group showed mild overexpression on the 1[st] day and no expression on other day posthepatectomy. The study demonstrated that TNF- alpha promoted the regenerative changes that occur in the liver after partial hepatectomy

INF-gamma, IL-5 and IGE profiles in chronic schistosomiasis mansoni Egyptian patients with or without hepatitis C infection

The immune response against clinical forms of chronic schistosomiasis mansoni patients with or without HCV infection was evaluated by assays the serum levels of IFN-gamma and IL- 5 for estimate the cell mediated immunity and IgE level to estimate the humoral immunity. This study included three patient groups. G.I included 25 patients with intestinal schistosomiasis, G.II included 15 patients with hepatosplenic schistosomiasis and G.III included 40 patients hepatosplenic schistosomiasis co-infected with HCV. Control G.IV included 15 healthy persons with matched age and sex. The intestinal group had high IFN-gamma [92%], normal level of IL-5 and IgE. The immune response was mainly 100% Th-1 response. The hepatosplenic patients had high IFN-gamma [26.7%], IL-5 [86.7%] and IgE [73.3%]. The immune response was 73.4% Th-0, 13.3% Th-l and 13.3% Th-2. The co-infected group had high IFN-gamma [62.7%], IL-5 [100%] and IgE [92.5%]. The immune response was 62.5% Th-0 and 37.5% Th-2 immunity. The shift to Th-0 and Th-2 immunity as well as associated depression of Th-1 in mixed group of patients may be playing a role in the persistence and severity of both diseases. Such immunity defects add to decrease challenge against HCV clearance

Assessment of CD5+B Cell Compartment in Chronic Hepatitis C virus patients

The present study was designed to assess the peripheral blood CD5+B lymphocytes in Egyptian patients with chronic HCV infection, which could help in predicting early autoreactivity and targeting appropriate therapeutic intervention. The present study is a cross-sectional analytical study carried out at the hepatology and gastroenterology unit of Suez-Canal University Hospital, Ismailia Egypt. Thirty individuals were enrolled in the study and classified into two subgroup; the study group 15 HCV-RNA positive associated chronic liver disease patients and the control group 15 adult apparently healthy volunteers blood donors. Individuals included in the study were subjected to medical history, clinical examination, complete liver function tests using the fully automated Hitachi-704 biochemical analyzer, serological tests for rheumatoid factor, HBV, HCV viral markers by Elisa technique, HCV-RT-PCR was used for detection of HCV RNA. ANA was tested by the indirect immunofluorescence technique, complete blood picture by the fully automated cell-day hematology counter and flowcytometric assessment of the peripheral blood CD19+/5+B lymphocytes by using B and D FACS caliber. The evident predominance of this B cell population in chronic liver disease patients with active HCV infection may give rise to immune-mediated squeal associated with HCV infection. This expanded population of CD5+B cells may modulate the course of the liver disease complicating HCV infection