ABSTRACT
Ginkgo biloba extract (GbE) has been indicated as an efficient medicine for the treatment of diabetes mellitus type 2. It remains unclear if its effects are due to an improvement of the insulin signaling cascade, especially in obese subjects. The aim of the present study was to evaluate the effect of GbE on insulin tolerance, food intake, body adiposity, lipid profile, fasting insulin, and muscle levels of insulin receptor substrate 1 (IRS-1), protein tyrosine phosphatase 1B (PTP-1B), and protein kinase B (Akt), as well as Akt phosphorylation, in diet-induced obese rats. Rats were fed with a high-fat diet (HFD) or a normal fat diet (NFD) for 8 weeks. After that, the HFD group was divided into two groups: rats gavaged with a saline vehicle (HFD+V), and rats gavaged with 500 mg/kg of GbE diluted in the saline vehicle (HFD+Gb). NFD rats were gavaged with the saline vehicle only. At the end of the treatment, the rats were anesthetized, insulin was injected into the portal vein, and after 90s, the gastrocnemius muscle was removed. The quantification of IRS-1, Akt, and Akt phosphorylation was performed using Western blotting. Serum levels of fasting insulin and glucose, triacylglycerols and total cholesterol, and LDL and HDL fractions were measured. An insulin tolerance test was also performed. Ingestion of a hyperlipidic diet promoted loss of insulin sensitivity and also resulted in a significant increase in body adiposity, plasma triacylglycerol, and glucose levels. In addition, GbE treatment significantly reduced food intake and body adiposity while it protected against hyperglycemia and dyslipidemia in diet-induced obesity rats. It also enhanced insulin sensitivity in comparison to HFD+V rats, while it restored insulin-induced Akt phosphorylation, increased IRS-1, and reduced PTP-1B levels in gastrocnemius muscle. The present findings suggest that G. biloba might be efficient in preventing and treating obesity-induced insulin signaling impairment.
Subject(s)
Animals , Male , Adiposity/drug effects , Dyslipidemias/drug therapy , Ginkgo biloba/chemistry , Obesity/drug therapy , Phytotherapy , Blood Glucose/analysis , Cholesterol, HDL/blood , Cholesterol, LDL/blood , Diet, High-Fat/adverse effects , Dyslipidemias/metabolism , Eating/drug effects , Glucose Tolerance Test , Hypoglycemia/blood , Insulin Receptor Substrate Proteins/analysis , Insulin Resistance/physiology , Insulin/metabolism , Muscle, Skeletal/chemistry , Obesity/etiology , Plant Extracts/therapeutic use , Protein Tyrosine Phosphatase, Non-Receptor Type 1/analysis , Proto-Oncogene Proteins c-akt/analysis , Rats, Wistar , Signal Transduction/drug effects , Triglycerides/bloodABSTRACT
The effect of the consumption of ethanol (5 percent) on retinol concentration in milk studied in the rat on day 12 after delivery, together with the evolution of dam body weight and pup growth rate. Female Wistar rats receiving alcohol (5 percent) in drinking water during lactation (N=7) were compared to normal controles fed ad libitum (N=6). The mean maternal alcohol intake was 3.96 + 0.23 g/kg body weight per day. To determine retinol levels in milk we used the Bessey and Lowry method, modified by Araújo and Flores (1978) Clinical Chemistry, 24:386-392). The pups were separated from dams for a 2-4h period, after which the dams were injected intraperitoneally with anesthetic and oxytocin. The concentration of retinol in milk was 162.88 + 10.60 mug/dl in the control group and 60.02 + 8.22 mug/dl in the ethanol group (P<0.05). The ethanol group consumed less food than the controls and lost a significant amount of weight during lactation. On days 8, 10 and 12, the body weight of the pups from rats given ethanol (13.46 + 0.43, 16.12 + 0.48 and 18.60 + 0.91 g, respectively) were significantly lower (P<0.05) than the weight of pups from controls (15.2 + 0.44, 18.36 + 0.54, 20.77 + 0.81 g). These data show that ethanol intake during the suckling period, even at low concentrations, decreases the amount of retinol in milk and, therefore, the amount available to the pups.