ABSTRACT
Candida albicans is an important human pathogen that can causes systemic infections, predominantly among populations with weak immune systems. Candida spp. produces biofilms on synthetic materials such as catheter, which facilitates adhesion of the organisms to devices. Since the frequent replacement of these devices is difficult and expensive to achieve solutions for rapid identification and control of biofilm infections is essential. The purpose of this study is, the use of zinc oxide nanoparticles in the manufacture catheter and evaluation of its inhibitory effect in preventing of the biofilm formation. In this experimental study, the catheters in making poly vinyl chloride, Znonano particles with aconcentration 2.027 microg/ml was used. Biofilms were formed over a series of time intervals [2, 4, 6, 8, 24 and 48 h]. At each time interval, biofilm formation was measured with the XTT and biomass assay and concurrently assessed by light microscopy. Results showed that catheters containing zinc oxide increases with time in cell density [absorbance increase] were lower than those without catheters. Similar results were also measured in dry weight. This study demonstrated antifungal activity of ZnO. ther for biofilm formation on medical equipment such as catheters, implants, dentures, artificial heart valves, etc can be inhibited by ZnO nanoparticles