Preparation, biodistribution and stability of [65 ZN] bleomycincomplex

Bleomycin [BLM] has been labeled with various radioisotopes and widely used in therapy and diagnosis. In this study BLM was labeled with [65Zn] zinc chloride and its distribution and stability in normal and tumor bearing mice was determined. The complex was obtained at the pH=2 in normal saline at 90 °C in 60 minutes. Radio-TLC showed an overall radiochemical yield of 95-97% [radiochemical purity >97%].The in vitro stability of the complex was determined in mice and human plasma. Preliminary studies were performed to determine distribution of [65Zn]BLM in normal and tumor bearing mice on the basis of these results. [65Zn]BLM may be used in therapeutic studies due to its suitable physico-chemical properties