Improvement of tissue survival of skin flaps by 5 alpha-reductase inhibitors: possible involvement of nitric oxide and inducible nitric oxide synthase

Skin flap grafting is a popular approach for reconstruction of critical skin and underlying soft tissue injuries. In a previous study, we demonstrated the beneficial effects of two 5alpha-reductase inhibitors, azelaic acid and finasteride, on tissue survival in a rat model of skin flap grafting. In the current study, we investigated the involvement of nitric oxide and inducible nitric oxide synthase [iNOS] in graft survival mediated by these agents. A number of 42 male rats were randomly allocated into six groups: 1, normal saline topical application; 2, azelaic acid [100 mg/flap]; 3, finasteride [1 mg/flap]; 4, injection of L-N[G]-nitroarginine methyl ester [L-NAME] [i.p., 20 mg/kg]; 5, L-NAME [20 mg/kg, i.p.] + azelaic acid [100 mg/flap, topical]; 6, L-NAME [20 mg/kg, i.p.] + finasteride [1 mg/flap, topical]. Tissue survival, level of nitric oxide, and iNOS expression in groups were measured. Our data revealed that azelaic acid and finasteride significantly increased the expression of iNOS protein and nitric oxide [NO] levels in graft tissue [P < 0.05]. These increases in iNOS expression and NO level were associated with higher survival of the graft tissue. It appears that alterations of the NO metabolism are implicated in the azelaic acid- and finasteride-mediated survival of the skin flaps

Knowledge and attitude towards stroke risk factors, warning symptoms and treatment in an iranian population

To assess the baseline knowledge regarding stroke risk factors, symptoms, treatment and information resources in an Iranian urban population. A community-based face-to-face interview survey was conducted in the Shiraz urban area in Iran. A total of 385 potential participants between the ages of 15 and 83 years were randomly selected from people referred to Motahari Clinic, Shiraz University of Medical Sciences, Shiraz. All the participants answered the 63 questions about different aspects of stroke. SPSS software version 15 was used to analyze the data. The 385 participants completed the face-to-face interview. The most common risk factors for stroke identified by respondents were hypertension [342; 88.8%] and smoking [338; 87.8%]. The most common warning signs of stroke were abdominal pain [370; 96.1%] and chest pain [338; 88.7%]. This study shows that the knowledge of and attitude towards stroke risk factors in the general population of Shiraz are adequate. By using the public media and school education, it is possible to promote the level of the population's knowledge of and attitude towards stroke