study on demodex folliculorum in rosacea

A random sample of 16 female patients suffering from papulopustular rosacea [PPR] as well as 16 normal female healthy subjects as control group was adopted in this study to assess of Demodex folliculorum pathogenesis. It was done through determination of mite density using a standard skin surface biopsy 10.5 cm2 from 6 different designated areas on the face, and scanning electron microscopic study [SEM] as well as total IgE estimation. A trial of treatment using Crotamiton 10% cream with special program was also attempted. All subjects' age ranged between 35 - 55 years old. All patients with rosacea and 15 of the control group [75.93%] were found to harbor mites. The mean mite counts by site distribution were 28.6 and 6.9 on the cheeks, followed by 14.5 and 3 on the forehead and lastly 6.8 and 0.8 on the chin in PPR and control groups, respectively. The total mean mite count in patients was 49.9 initially and 7.9 after treatment. In the control group, it was 10.7 and 10.6, respectively. The mean total IgE was 169.4 and 168.4 as well as 96.3 and 98.4 in PPR and control groups, respectively. Light and scanning electron microscopy revealed that all mites were pointing in one direction. Some of them were containing bacteria inside their gut and on their skin. After treatment, three cases were completely cured, ten cases gave moderate response, while three cases showed no response. In conclusion, this study supported the pathogenic role of D. folliculorum in rosacea

Synthesis of certain sulfones, sulfanilamides and sulfonamides of pyrazolo [5,4-b] imidazo [2,1-b] thiazole with antibacterial activity

6-phenylimidazo [2, 1-b] thiazole-3-N, N-dimethylacetamide [I] was formulated using Vielsmeier reagent to afford the corresponding 5-formyl derivative [II]. Treatment of II with benzenediazonium chloride gave 5-formyl-6-phenylimidazo [2, 1-b] thiazole-3-[N, N- dimethyl-1-phenylazo] acetamide [III]. Cyclization of III using FeCl3 and DMSO or by heating in nitrobenzene afforded the corresponding new fused system, 3-N, N-dimethylacarbamoyl-1, 6-diphenylpyrazolo [5, 4-b] imidazo [2, 1-b] thiazole-5-carboxaldehyde [IV]. The aldehydic function in IV was converted into substituted sulfones, sulfanilamides and sulfonamides and the antibacterial activity of the newly prepared compounds was evaluated