Expression of cytosolic and thiolated proteome of Musca domestica larvae under oxidative challenge

@#The study was aimed to investigate the expression of cytosolic and thiolated proteins of Musca domestica larvae under oxidative stress. Proteins from acute treatment of hydrogen peroxide (LC50 = 21.52% (v/v)) on 3rd stage larvae of housefly were extracted and purified using an activated Thiol Sepharose® for thiolated protein purification. Two dimensional gel electrophoresis was used for visualizing and analyzing expression of cytosolic and thiolated proteins. Protein spots with more than 5 fold of expression change were identified using liquid chromatography- tandem mass spectrometry (LC-MS/MS). The cytosolic proteins were actin, tropomyosin, ubiquitin, arginine kinase, pheromone binding protein/general odorant binding protein, and ATP: guanidino phosphotransferase. The thiolated proteins with more than 5 fold change in expression as an effect to the acute treatment were fructose bisphosphate aldolase, short chain dehydrogenase and lactate/malate dehydrogenase. The proteins identified in the study should provide vital information for future reference in oxidative stress defence and response occurring in houseflies.

Purification, expression and partial characterization of glutathione s-transferases (GSTs) from three different strains of Aedes albopictus (Diptera: Culicidae)

Glutathione S-transferases (GSTs) are multifunctional enzymes involved in detoxification of xenobiotic compounds in many insect groups. This work investigated the expression of GSTs in Aedes albopictus by using affinity chromatography and proteomic analysis. The study compared the expression of GSTS isoforms in larvae of three Ae. albopictus strains (susceptible (VCRU) strain, field Kuala Lumpur (KL) strain and laboratory permethrintreated strain). The study demonstrated that the expression of sixteen isoforms (GSTS1-1, GSTS1-4, GSTS1-3, GSTS1-4, GSTD1-3, GSTD1-2, GSTD1-5, GSTD1-6, GSTD1-7, GSTD1-8, GSTD1-9, GSTD1-10, GSTD1-11, GSTT, GSTD1-12, and GSTD1-13) were significantly increased in the field KL strain (p0.05). However, while comparing the expression of GST isoforms in field KL larvae and laboratory permethrin-treated strain, the same pattern was observed for all the GSTs especially for Theta and Sigma class (p>0.05). In Delta class, only GSTD1-1, GSTD1-9, GSTD1-11, GSTD1- 12 and GSTD1-13 shows significant difference in expression between these two strain (p<0.05). This comparative data on GST expression in Ae. albopictus can be useful database to identify possible underlying mechanisms governing insecticide resistance by GSTs.