ABSTRACT
Objective: To investigate effects of intraperitoneally administration of alpha-tocopherol loaded nanoparticles [TNP] on ischemia-reperfusion injury in ovaries
Methods: Thirty-five healthy female Wistar rats 250g were randomized into seven experimental groups [n=5]: Group SHAM: The rats underwent only laparotomy. Group Ischemia: A 3- hour ischemia only. Group I/R: A 3-hour ischemia and a 3-hour reperfusion. Group I/T: A 3-hour ischemia only and 100 mg/kg intraperitoneal administration [IP] of alpha-tocopherol 2.5 hours after induction of ischemia. Group I/R/T: A 3-hour ischemia, a 3-hour reperfusion and 100 mg/kg IP of alpha-tocopherol 2.5 hours after induction of ischemia. Group I/TNP: A 3-hour ischemia only and 1 mg/kg IP of TNP 2.5 hours after induction of ischemia. Group I/R/TNP: A 3-hour ischemia, a 3-hour reperfusion and 1 mg/kg IP of TNP 2.5 hours after induction of ischemia
Results: Animals treated with alpha TNP showed significantly ameliorated development of ischemia and reperfusion tissue injury compared to those of other groups [p=0.001]. The significant higher values of SOD, tGSH, GPO, GSHRd and GST were observed in I/R/NC animals compared to those of other groups [p=0.001]. Damage indicators [NOS, MDA, MPO and DNA damage level] were significantly lower in I/R/NC animal compared to those of other groups [p=0.001]
Conclusion: Intraperitoneal administration of TNP could be helpful in minimizing ischemia-reperfusion injury in ovarian tissue exposed to ischemia
ABSTRACT
Objective: to assess the effect of heparin binding neurotrophic factor [HBNF] on sciatic nerve regeneration in animal model of rat
Methods: seventy-five male Wistar rats were divided into five experimental groups randomly [each group containing 15 animals]: Sham operation group [SHAM], autograft group [AUTO], transected control [TC], chitosan conduit [CHIT] and heparin binding neurotrophic factor treated group [CHIT/HBNF]. In AUTO group a segment of sciatic nerve was transected and reimplanted reversely. In SHAM group sciatic nerve was exposed and manipulated. In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle [TC]. In treatment group defect was bridged using a chitosan conduit [CHIT] filled with 10 [micro]L [0.1 mg/mL] HBNF [CHIT/HBNF]. Each group was subdivided into four subgroups of five animals each and nerve fibers were studied in a 12-week period
Results: behavioral, functional, biomechanical, electrophysiological and gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in treatment group than in CHIT group [p=0.001]. Immunohistochemical reactions to S-100 in treatment group were more positive than that in CHIT group
Conclusion: local administration of HBNF improved functional recovery and morphometric indices of sciatic nerve. It could be considered as an effective treatment for peripheral nerve repair in practice
ABSTRACT
Objective: To assess the neuroprotective effects of local administration of 17- beta- estradiol on nerve regeneration
Methods: Sixty female Wistar rats were overiectomized and divided into four experimental groups [n = 15], randomly: In autograft group a segment of sciatic nerve was transected and re-implanted reversely. In sham-surgery group sciatic nerve was exposed and manipulated. In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle. In treatment group defect was bridged using a silicon conduit filled with 10 microL [0.1 mg/mL] 17- beta- estradiol. Each group was subdivided into four subgroups of five animals each and nerve fibers were studied in a 12-week period
Results: Behavioral, functional, biomechanical, electrophysiological and gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in treatment group than in other groups [p<0.05]. Immunohistochemical reactions to S-100 in treatment group were more positive than that in other groups
Conclusion: Local administration of 17-beta-estradiol improved functional recovery and morphometric indices of sciatic nerve. It could have clinical implications for the surgical management of patients after facial nerve transection
ABSTRACT
Currently, chemotherapy and radiotherapy and considered most effective methods for cancer treatment, however these strategies often result in fertility problems. A favorable alternative to prevent fertility loss in cancer patients is the cryopreservation and transplantation of sexual tissues [ovaries and/or testes]. There is a low rate of fertilization following cryopreservation of ovaries prior to implantation. Therefore, in our opinion, this low rate is caused by instable blood flow during organ transplantation. Thus, this study researches a canine ovarian model that focuses on direct exposure of ovaries with blood in an experimentally induced sinus-like cavity. We implanted this tissue on the muscular layer of the stomach, which is its most vascularized region. Ovarian transplantation was conducted on T1 animals [n=5], bilateral ovariectomy was performed on T2 animals [n=5], unilateral ovariectomy was conducted on T3 cases and animals in the control-sham group [n=5] did not undergo ovariectomy or transplantation. All isotransplanted ovaries survived. Ovaries resumed follicular growth and revascularization. Transplanted ovaries contained 75%-76% of survived small follicles [pre antral] after 60 days. The ovarian granulosa cells showed considerable resistance against ischemia. After day 30 no statistically significant differences in the level of estradiol and progesterone were observed between T1 animals and the T3 group. T1 animals showed considerably high level of progesterone and estradiol in comparison to T2 cases. This study showed that using blood sinus method for ovarian isotransplantation helps ovarian tissue to survive from post implantation ischemia which confirms with normal follicles presentation and intact endocrine function of the implanted ovaries