ABSTRACT
Aims: The objective of this work was to analyze the anti-inflammatory, cytotoxic and antioxidant activity of the fractions of ethyl acetate from the leaves of D. pubipetala. Methodology: Liquid-liquid partitioning was performed, the ethyl acetate partition was eluted in classical liquid chromatography, followed by thin layer chromatography. Antioxidant activity was performed by the DPPH photochlorimetric method and the levels of interleukin (IL?6), tumor necrosis factor ? (TNF??), interleukin-10 (IL-10) and nitric oxide (NO) were identified in supernatants of cell cultures. Results: Fractions F4, F5 and F10 showed antioxidant activity. The F10 fraction indicated efficient anti-inflammatory activity by the production of IL-10 and nitric oxides. Fractions F4, F5 and F9 stimulated the production of IL-6 and TNF-?. Conclusion: These results indicate a possible synergistic action of the extract and the partition of ethyl acetate, since after fractionation the potential presented in previous studies was lower.
ABSTRACT
Abstract This study was developed to evaluate the effect of seasonality on the yield and chemical composition of the essential oil (EO) of Hesperozygis ringens (Benth.) Epling, a native species from the Brazilian Pampa. Leaves were collected from four specimens of a single population in each of the four seasons for a year and were extracted in triplicate by hydro-distillation for 2 hours. The yield of EO (% w/w) was calculated on fresh weight basis (FWB), and the 16 oil samples were analyzed by gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detector (GC-FID). Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA) were used as statistical tools to evaluate differences in chemical composition. The highest yields were obtained in autumn, spring and summer (2.32-4.38%), while the lowest yields were detected in winter, ranging from 1.15 to 1.91%. Oxygenated monoterpenoids were the predominant class of chemical constituents in the EO obtained in all seasons, showing the highest contents in autumn and summer, and pulegone was identified as a major compound, whose contents varied between 54.13 and 81.17%. The EO samples were divided into three chemical groups by HCA and PCA and were assigned to the same group, except for the three samples gathered in winter. The results showed a seasonal influence on the yield and chemical composition of the EO.
Resumo Este estudo foi desenvolvido a fim de avaliar o efeito da sazonalidade no rendimento e composição química do óleo essencial (OE) de Hesperozygis ringens (Benth.) Epling., uma espécie nativa do Pampa brasileiro. Folhas foram coletadas de quatro indivíduos de uma mesma população, em cada uma das quatro estações de um ano, e foram extraídas em triplicada por hidrodestilação durante 2 horas. O rendimento do OE (% m/m) foi calculado considerando a base fresca (BF) e as 16 amostras de óleo foram analisadas por cromatografia gasosa acoplada à espectrometria de massas (CG-EM) e cromatografia gasosa com detector de ionização de chamas (CG-DIC). Análise Hierárquica de Cluster (AHC) e Análise de Componentes Principais (ACP) foram utilizadas como ferramentas estatísticas para avaliar as diferenças na composição química. Os maiores rendimentos foram obtidos no outono, primavera e verão (2,32-4,38%), enquanto que os menores foram detectados no inverno, variando de 1,15 até 1,91%. Os monoterpenoides oxigenados foram a classe predominante dos constituintes do OE obtido em todas as estações, apresentando os maiores teores no outono e no verão, e a pulegona foi identificada como o constituinte majoritário, cujos teores variaram entre 54,13 e 81,17%. As amostras de OE foram divididas em três grupos químicos por AHC e ACP e foram classificadas no mesmo grupo, com exceção de três amostras coletadas no inverno. Os resultados demonstraram influência sazonal no rendimento e na composição química dos OE.
Subject(s)
Lamiaceae/chemistry , Lamiaceae/metabolism , Oils, Volatile/chemistry , Oils, Volatile/metabolism , Cluster Analysis , Flame Ionization , Gas Chromatography-Mass Spectrometry , Principal Component Analysis , Plant Leaves/chemistry , SeasonsABSTRACT
No período de janeiro a outubro de 2007, foram tuberculinizadas 1.866 fêmeas caprinas de diferentes raças e idades distribuídas em 84 propriedades da microrregião de Monteiro, semiárido da Paraíba. Nove cabras (0,48%) foram positivas, duas delas foram sacrificadas e, à necropsia foram observadas lesões sugestivas de tuberculose nos pulmões e no fígado. No exame histopatológico, foram evidenciados granulomas típicos. Foi procedido o cultivo e isolamento de micobactérias, sendo observado o crescimento do agente no meio de cultura de Stonebrink a partir de 36 dias de incubação, com um tempo médio de crescimento de 42 dias. Também foram encontrados resultados positivos na pesquisa de bacilos álcool-ácido resistentes (BAAR) pela coloração de Ziehl-Neelsen. O cultivo de Corynebacterium pseudotuberculosis foi negativo. O presente relato, com isolamento e visualização do agente, bem como verificação de lesões macro e microscópicas, é o primeiro caso de tuberculose confirmado em caprinos do semiárido brasileiro.
From January to October 2007, 1,866 female goats of different breeds and ages from 84 herds of the Monteiro microregion, in a semiarid area of Paraíba State, Brazil, were tuberculinized. Nine goats (0.48%) were positive and two of them were slaughtered, and at necropsy tuberculosis-like lesions were found in lungs and liver. At the histopathologic examination, typical tuberculosis granulomas were observed. Micobacteria culture and isolation were carried out, and there was growth of the agent on the Stonebrink medium culture from 36 days of inoculation with an average growth time of 42 days. Positive results were also found in the research of acid-fast bacilli by Ziehl-Neelsen staining. The culture of Corynebacterium pseudotuberculosis was negative. This report, with isolation and visualization of the agent, as well as verification of gross and microscopic lesions, is the first confirmed case of tuberculosis in goats of a semiarid region of Brazil.
Subject(s)
Animals , Female , Tuberculosis/veterinary , Goats/microbiology , Corynebacterium pseudotuberculosis/isolation & purificationSubject(s)
Humans , Male , Female , Middle Aged , Osteoporosis/etiology , Osteoporosis/drug therapy , Causality , Bone Density/physiology , Fractures, Bone/etiology , Osteoporosis, Postmenopausal/drug therapy , Argentina , Bone and Bones/physiology , Bone and Bones/physiopathology , Sex Distribution , Osteoporosis, Postmenopausal/etiology , Osteoporosis, Postmenopausal/physiopathology , Bone Resorption/diagnosis , Bone Resorption/diet therapy , Bone Resorption/prevention & controlABSTRACT
Dois casos de falecimento em provas ergométricas, com a particularidade de que se realizou coronariografia imediatamente depois do acidente agudo. Ambos apresentavam lesöes na artéria coronária esquerda, de tipo excêntrico, com bordas irregulares e brilho intraluminal. Concluí-se que a rotura da placa ateromatosa foi acontecimento fundamental na patogênese deste fenômeno
We present two cases of mortality during a stress test after which coronary arteriography was performed after the acute event. In both patients, left coronary arteries presented eccentric lesions with irregular bordes and intraluminal lucencies. We have discussed the probable pathophysiologic mechanisms involved in view of the angiographic findings. We concluded that the cracking of the plaque was an essential event in the pathogenesis of this picture.
Subject(s)
Humans , Male , Middle Aged , Coronary Disease/physiopathology , Death, Sudden/etiology , Exercise Test/adverse effects , Coronary VesselsABSTRACT
Se presentan los resultados de un estudio cooperativo sobre la exploracion de la via biliar con 99mTc diisopropil-IDA. Se estudiaron 15 voluntarios normales y 75 pacientes portadores de colecistitis aguda (CA), y cronica (CC), ictericias hepatocelulares (IHC), obstructivas coledocianas incompletas (ICI) y completas (ICC).Se determino el comportamiento biologico del radiofarmaco en los dos grupos estudiados a traves de la medida de los tiempos de captacion y aparicion en el parenquima hepatico, en la via biliar principal intra y extrahepatica, en la vesicula, en el duodeno y en los rinones. Se describen los modelos fisiopatologicos encontrados en la CA, CC, IHC, ICI, ICC. Se concluye que el procedimiento tiene indicacion de eleccion en la CA, que su valor es limitado en la CC y que en las ictericias salvo en situaciones muy especiales el metodo aporta muy poca informacion de utilidad clinica. Se destaca la posibilidad que Servicios Latinoamericanos realicen trabajos cientificos en forma conjunta acrecentando su nivel de eficacia
Subject(s)
Infant , Adolescent , Adult , Middle Aged , Humans , Male , Female , Cholelithiasis , Cholestasis , Radionuclide Imaging , Imino Acids , TechnetiumABSTRACT
En 7 casos con hernia hiatal comprobada radiologicamente se realizo centellograma esofagogastrico. Se describe la tecnica utilizada. En 6 se obtuvo evidencia centellografica de reflujo, mientras que la radiografia simple habia detectado 4. Resulta un metodo confortable para los pacientes ya que elimina el uso de la sonda nasogastrica