A simple method for human peripheral blood monocyte isolation

We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.

Histoplasmona com derrame pleural: relato de um caso / Histoplastoma with pleural effusion: report of a case

Efusöes pleurais surgem raramente em associaçäo com a histoplasmose capsulata, ocorrendo em geral nas formas agudas da doença. Relatamos e discutimos um caso clínico em que um histoplasma subpleural acompanhou-se de dor pleurítica, hidrotórax e pleurite fibrosante