ABSTRACT
The ultimate goal of any peripheral nerve repair is maximum sensory and motor recovery of the distal denervated part. Conventional suture repair may result in neuroma formation and a fibrotic reaction that is detrimental to nerve regeneration. Further studies concerning laser and its effect on the peripheral nerves in rats and other species have helped in the development of laser-assisted nerve anastomosis. In the field of otorhinolaryngology, facial nerve anastomosis, particularly in difficult-to-reach sites, may need such a non-contact sutureless laser technique to overcome the difficulties of other conventional methods. The aim of this work is to assess the effect of CO[2] laser as a tool for sciatic nerve welding in rats [with and without solder] and comparing it with the conventional suture repair. Sixty rats were used in this study classifying into 5 Groups: Group A: 12 rats for laser nerve welding without solder. Group B: 12 rats for laser nerve welding with solder. Group C: 12 rats for nerve anastomosis with sutures. Group D: 12 rats for delayed laser nerve welding without solder [one week after cut]. Group E: 12 rats for delayed laser nerve welding without solder [two week after cut]. After follow-up period of 8 weeks the rats of each group were assessed physically for functional recovery [motor and sensory], elecrtophysiologically for nerve conduction and histopathologically for morphological study of nerve fibers. It was found that no significant difference between laser groups and suture group regarding physical, electrophysiological and histopathological results. Only the dehiscence rate was higher in laser groups than in suture group. The soldered laser group was the least one concerning dehiscence. It is concluded that CO[2] laser -assisted nerve welding is as effective as microsuturing technique, but easier, less tedious, less time consuming and easy to reach difficult areas