ABSTRACT
Background: Hormonal control for multiple follicular growths in IVF program was directed toward maximizing the yield of fertilizable oocytes, enhance successful implantation and improve early embryonic development
Aim: The effect of different doses of urinary human menopausal gonadotropin [U-hMG] in superovulation regimen was studied
Materials and Methods: Four groups of immature female mouse were sequentially injected by 5 IU, 10 IU, 15 IU and 20 IU of U-hMG followed by injection of 15 IU human chorionic gonadotropin [hCG] for all groups. Mature cumulus oocytes complexes [COCs] were collected 12 hours later then the grading of cumulus cell expansion and oocytes maturity was determined. In vitro fertilization for mature oocytes was done by using epididymal sperm, the embryonic development was followed up till the blastocyst stage
Results: Results demonstrated that the super ovulatory response in immature mice increased with increasing dose of hMG. 15 IU U-hMG was the optimal dose that gave maximum number oocytes with higher maturation rate,minimum degeneration rate and support embryonic development
Conclusion: 15 IU is the optimal dose for induction of superovulation and support in vitro embryo developmentin immature female mice
ABSTRACT
Background: Oxidative stress is known to be a key factor in several diseases and was reported as a result of radiation exposure in human and experimental animals. Borage [Borago officinal L.] oil contains a high percentage of g-linolenic acid, which reported as a preventive treatment of a wide range of disorders
Aim: The present study was designed to determine the possible protective effects of borage seeds oil against gamma [gamma-] radiation induced oxidative stress in male rats
Materials and Methods: Thirty rats were divided into five groups [6 rats each]: Control, irradiated; rats were exposed to [6.5 Gy] of whole body gamma-radiation, borage seeds [50 mg/kg b.wt], irradiated borage treated pre-irradiated and borage treated post-irradiated. Serum aspartate aminotransferase [AST], alanine aminotransferase [ALT], gamma glutamyl transferase [GGT] levels, as well as serum and hepatic reduced glutathione [GSH], superoxide dismutase [SOD] and lipid peroxide [malondialdehyde] [MDA] levels were assessed
Results: The results showed that, activities of GSH were highly significant decrease, while marked highly significant increase in MDA due to irradiation. Supplementation with BO before or treated after irradiation, exerted marked amelioration of irradiation induced disturbances in all the investigated parameters. Therefore, borage could have a beneficial role in modulating oxidative stress by improving the natural antioxidant mechanism
Conclusion: borage has a beneficial role in reducing hepatotoxicity and oxidative stress induced by radiation exposure
ABSTRACT
Introduction: exposure to ionizing radiation represents a genuine, increasing threat to mankind and our environment. The steadily increasing applications of radiation in clinical practice, industrial and agricultural activities, on top of residual radioactivity resulting from nuclear test explosions, have a measurable impact contributing to possible radiation hazards in humans. Control of radiation hazards is considered as one of the most important challenges in order to protect our lives from radiation damages. The trans-3,4,5-trihydroxystibeneis a phyto-chemical present in peanuts and grapes with beneficial effects such as protection against cardiovascular disease and cancer prevention
Purpose: the present study aims to clarify the role of peanut oil as a radioprotector in male albino rats against oxidative stress and bone injury induced by two different doses of gamma irradiation
Material and Methods: rats were subjected to a dose of 5 Gy [group 3] or 10 Gy[group 4] [single dose/ whole body] in comparison with control group [group 1]. Prior to the two doses of gamma radiation, rats received peanut oil subcutaneously, [0.75 ml/kg] over one month period, on three days/week [group 5 and 6]. Group 2, rats received peanut oil subcutaneously, [0.75 ml/kg] as group 5, but without exposure to radiation
Results: the results showed that whole body gamma irradiation revealed significant acceleration in the level of lipid peroxide [MDA], with significant depletion in glutathione content [GSH], superoxide dismutase [SOD] and catalase [CAT] activities. Meanwhile. Also, the study showed significant increase in serum calcium with concomitant decrease in the bone calcium and significant increase in serum inorganic phosphorous concomitant with a decrease in bone phosphorous after radiation exposure. Administration of peanut oil pre-irradiation has significantly ameliorated the radiation induced disturbance in all the investigated parameters
Conclusion: Metabolism can be controlled to some extent by peanut oil administrated prior to irradiation
ABSTRACT
Background: the present study aimed to investigate the dynamics of immunoregulatory factors implicated in the murine model of soluble adult worm antigen preparation [SWAP] and UV-irradiated adult worm extract-induced granulomatous hypo-responsivness
Material and Methods: native male mice were classified into four groups. The first negative control [non-infected] group, the second positive control [infected] group, the third SWAP-vaccinated group and the fourth UV-irradiated adult worm extract-vaccinated group. The last two groups were injected subcutaneous by 0.2 ml [contained 100 µg protein] of the two used vaccines SWAP or UV-irradiated adult worm extract respectively, four weeks prior to infection. The 2nd, 3rd and 4th mice groups were infected by subcutaneous injection with 100 cercariae of Egyptian strain of S. mansoni. All mice groups were sacrificed 8 weeks post-infection
Results: parasitological parameters such as worms count, eggs/gram tissues of liver and intestine were studied. Biochemical parameters such as the activities of liver function tests [AST, ALT, ALP and [Gamma]GT] were measured. The cellular immune responses were assessed by studying the cytokine measurement. Hepatic histopathological criteria and the morphological changes of adult worms were studied through the scanning electron microscope
Conclusion: the present results revealed that the UV-irradiated adult worm extract have high efficacy than SWAP as immunizing antigens as shows in our data, which reported that irradiation associated vaccine antigen was shown to achieve a higher protection in mice