ABSTRACT
Prevalence of CMV and toxoplasmosis antibodies were determined among normal newborn infants, suspected congenital neonates and pregnant women. Seropositive rates of CMV and toxoplasmosis were similar. Most of the pregnant women had CMV antibodies. The in-house ELISA for detection of Toxoplasma antibodies was developed and compared with the commercial kit with sensitivity and specificity of 90.47% and 96.74% respectively.
Subject(s)
Animals , Antibodies, Protozoan/blood , Antibodies, Viral/blood , Cytomegalovirus/immunology , Cytomegalovirus Infections/congenital , Enzyme-Linked Immunosorbent Assay , Female , Humans , Infant, Newborn , Pregnancy , Seroepidemiologic Studies , Thailand/epidemiology , Toxoplasma/immunology , Toxoplasmosis, Congenital/immunologyABSTRACT
Immunostaining was compared with PCR for diagnosis of congenital CMV infection. IgM and IgG antibody assays were also performed in parallel. Immunostaining gave sensitivity and specificity of 60% and 97% respectively. Correlations among immunostaining, PCR and the presence of IgM antibody was reported. Immunostaining can be used for early diagnosis of congenital CMV infection in parallel with detection of IgM antibody.
Subject(s)
Base Sequence , Cytomegalovirus Infections/congenital , DNA Primers , Enzyme-Linked Immunosorbent Assay , HeLa Cells , Humans , Immunoglobulin G/immunology , Immunoglobulin M/immunology , Infant, Newborn , Neonatal Screening , Polymerase Chain Reaction , Sensitivity and Specificity , ThailandABSTRACT
Four hundred and forty-one blood and serum samples were collected during August to October 1998 from the blood donors at the blood bank of Rajvithi Hospital, Bangkok, Thailand. Their ages were varied between 18-55 years. All specimens were tested by immunostaining and ELISA methods. Forty-seven specimens (10.66%) gave positive results by immunostaining. Among these, 20 cases were seropositive and 27 cases were seronegative. The age group between 41-50 years had a high percentage of CMV infection as judged by the immunostaining method, more than the other age groups. By ELISA, 231 cases (52.38%) had positive IgG antibody to CMV, 42 cases (9.52%) were IgM antibody positive and 39 cases (8.84%) were positive for both IgG and IgM antibodies. The age groups between 36-40 years had a higher percentage of IgM antibody positives than the other age groups. Since the immunostaining method can detect early CMV infection, screening for the presence of antibodies alone is not enough to rule out CMV infection. Immunostaining along with ELISA detection of antibodies was useful for determining a decrease in CMV infection.