ABSTRACT
Objective: To determine the effects of bone marrow derived mast cells [BMMCs] on functional recovery of transected sciatic nerve in animal model of cat
Method: A 20-mm sciatic nerve defect was bridged using a silicone nerve guide filled with BMMCs in BMMC group. In Sham-surgery group [SHAM], the sciatic nerve was only exposed and manipulated. In control group [SILOCONE] the gap was repaired with a silicone nerve guide and both ends were sealed using sterile Vaseline to avoid leakage and the nerve guide was filled with 100 microL of phosphate-buffered saline alone. In cell treated group [[SILOCONE/BMMC] the nerve guide was filled with 100 microL BMMCs [2× 106 cells/100 microL]. The regenerated nerve fibers were studied, biomechanically, histologically and immunohiscochemically 6 months later
Results: Biomechanical studies confirmed faster recovery of regenerated axons in BMMCs transplanted animals compared to control group [p<0.05]. Morphometric indices of the regenerated fibers showed that the number and diameter of the myelinated fibers were significantly higher in BMMCs transplanted animals than in control group [p<0.05]. In immunohistochemistry, location of reactions to S-100 in BMMCs transplanted animals was clearly more positive than that in control group
Conclusion: BMMCs xenotransplantation could be considered as a readily accessible source of cells that could improve recovery of transected sciatic nerve