Effects of the hydro-ethanolic extract of Marrubium vulgare in female rats

OBJECTIVE@#To evaluate the effects of ethanol-water (80:20) extract of Marrubium vulgare (M. vulgare) on the hematological parameters, macroscopic and histological aspects of the uterus and fetus in non-pregnant and pregnant rats.@*METHODS@#Female rats were divided into 4 equal groups (n = 9), group N (normal rats) and group G (pregnant rats) considered as control groups, group NE (normal rats treated with the ethanol-water (80:20, v/v) extract of M. vulgare) and group GE (pregnant rats treated with the extract). The ethanol-water (80:20) plant extract was administered in a single daily dose 1 g/kg at the morning, during 19 d. On the 19 day of the experiment, animals were sacrificed, the uterus and fetuses were removed for the morphological and histological studies and the blood was collected in EDTA tubes for the measurement of hematological parameters with the use of an automate 'HORIBA ABX Micros 60 Hematology Analyzer'.@*RESULTS@#Our results showed, in group NE and GE, a significant decrease on hematological parameters: red blood cells (NE: 18.6%; GE: 38.4%), hematocrit (NE: 13.8%; GE: 20.4%), hemoglobin (NE: 12.1%; GE: 8.3%) and mean corpuscular volume (NE: 6.4%; GE: 2%) with P more less a 0.05. Indeed, the extract of M. vulgare caused a significant decrease on the mean implantations of fetuses (82.5%, P < 0.001) and their size (47.2%, P < 0.01). As for the macroscopic and histological appearance of uterus, our data showed no change in normal treated rats. In contrast, the treated pregnant rats showed a severe histological change characterized by the existence of location of stopped gestation. Furthermore, it was also found in the uterus of these rat lyses placental and embryo tissue.@*CONCLUSIONS@#All these results support the hypothesis of an abortifacient effect of M. vulgare.

Effects of the hydro-ethanolic extract of Marrubium vulgare in female rats

Objective To evaluate the effects of ethanol–water (80:20) extract of Marrubium vulgare (M. vulgare) on the hematological parameters, macroscopic and histological aspects of the uterus and fetus in non-pregnant and pregnant rats. Methods Female rats were divided into 4 equal groups (n = 9), group N (normal rats) and group G (pregnant rats) considered as control groups, group NE (normal rats treated with the ethanol–water (80:20, v/v) extract of M. vulgare) and group GE (pregnant rats treated with the extract). The ethanol–water (80:20) plant extract was administered in a single daily dose 1 g/kg at the morning, during 19 d. On the 19 day of the experiment, animals were sacrificed, the uterus and fetuses were removed for the morphological and histological studies and the blood was collected in EDTA tubes for the measurement of hematological parameters with the use of an automate ‘HORIBA ABX Micros 60 Hematology Analyzer’. Results Our results showed, in group NE and GE, a significant decrease on hematological parameters: red blood cells (NE: 18.6%; GE: 38.4%), hematocrit (NE: 13.8%; GE: 20.4%), hemoglobin (NE: 12.1%; GE: 8.3%) and mean corpuscular volume (NE: 6.4%; GE: 2%) with P more less a 0.05. Indeed, the extract of M. vulgare caused a significant decrease on the mean implantations of fetuses (82.5%, P < 0.001) and their size (47.2%, P < 0.01). As for the macroscopic and histological appearance of uterus, our data showed no change in normal treated rats. In contrast, the treated pregnant rats showed a severe histological change characterized by the existence of location of stopped gestation. Furthermore, it was also found in the uterus of these rat lyses placental and embryo tissue. Conclusions All these results support the hypothesis of an abortifacient effect of M. vulgare.

[Profile of biochemical markers in cystic fibrosis. prospective study about 13 cases]

To study the disturbances of biochemical parameters which make it possible to evaluate the nutritional state during the cystic fibrosis. Prospective study about 13 cases hospitalized over a 7 months period, whose age varies between 2 months and 12 years and adressed for a suspicion of cystic fibrosis. For all the patients we made a clinical collection of the data and a biochemical study. Hypoprotidemia [protidemia < 60 g/l] was noted in 6 cases. Three cases presented a hypocalcaemia [calcemia < 2.20 mmol/l]. A hypomagnesaemia [magnesemia < 0.70 mmol/L] was observed in 2 cases. The totality of the patients presented zinc concentrations lower than the normal value. Iron concentrations lower than the normal [11- 24 mmol/l] was noted in 5 cases. Four patients [4/13] presented a concentration of iron between 11.2 and 20 mmol/l, whereas a high concentration [32.3 mmol/l] was noted in only one case [7.7%]. The study of the biochemical parameters allowed to evaluate the variation of some trace elements in cystic fibrosis and their consequence on the nutritional state of the patients, which constitutes an essential element for the assessment of these patients