Discussing the stabilizing bacteriorhodopsin through spin coating method in protein nano-memory

Protein memories are novel technologies which use the hidden abilities of bacteria proteins and biological molecules in order to the electronic processes. Four decades before baceriorhdopsin [ BR] protein was extracted from the membrane of Halobacerinum Salina rum found in high salt water depositions after its original discovery in 1967. In this study, for immobilizing the protein a film has been provided from the different weight/volume use of polyvinyl alcohol and gelatin polymers. Then a suspension of protein bacteriorhodopsin with the 3/2 mg.ml [-1] has been used that according to the spectroscopy and determining their activity, optimizing concentration of a film containing bacteriodospian has been obtained. Polymeric film containing bacteriorhodopsin protein has been injected on the glass and by use of spin coater, after obtaining optimizing protocol become stabilized. After that the biological activity of the bacteriorhodopsin protein has been studied. Several biological molecules are being considered based on their usage in computers sciences, as though the bacterial protein - BR has generated much interest among scientists. Functional immobilization of membrane proteins requires consideration of their physiological needs, often dictated by the quality and components of the natural hydrophobic environment surrounding this class of proteins

Surface modification by using of immobilized electrostatic self-assembly of bacteriorhodopsin as protein memory

Bacteriorhodopsin [BR] is the light harvesting and photoactive proton pump found in the membrane of a salt marsh bacteria. This protein has significant potential to use in optical computing and memory devices due to unique intrinsic physical properties of photo and bioelectric. All these features make BR one of the most promising protein candidates in protein memories. Protein memory is a kind of optical memory with a large storage capacity and high speed processing features. BR protein was used with the polymer film in order to create better stability. In order to investigate immobilization of electrostatic self-assembly of BR on glass and polycarbonate as protein memories was used. Polycarbonate is a layer of compact disc [CD] structure which considered dye immobilized on its surface and have reading and writing abilities of information via 0,1 bites. In this study, surfaces of polycarbonate modified by the mixture of 5% sulfuric acid and 20% acetic acid; furthermore, by using of PEI as cationic resin the surface of polycarbonate was charged and BR immobilized on it electrostatically. The modified surfaces were characterized by AFM technique. Also, light activity for reading data is retained. This is an appropriate method for optimal stability and activity assay of the protein and also is suitable for preparation of protein memories