ABSTRACT
Chronic lymphocytic leukaemia [CLL] is the most common B-cell malignancy in the western world and exists as subtypes with very different clinical courses. Myeloid cell leukemia 1 [McL[-1]] is one member of Bcl-2 family proteins that has been shown to be expressed in various tissues and malignant cells, including CLL, where its expression is significantly associated with a failure to achieve complete remission following cytotoxic therapy. Induction of apoptosis by prenylated coumarin, umbelliprenin, in Jurkat cells was previously shown. We examined whether umbelliprenin can down-regulate McL[-1] gene and protein in Jurkat cells. In this regard cells were incubated by umbelliprenin, and then down- regulation of McL[-1] gene was studied by Real Time PCR method. Moreover, down-regulation of McL[-1] protein was studied by western blot analysis. We showed that, expression of McL[-1] mRNA was increased from 1 hour to 3 hours incubation, but this increase has a scale down pattern. Moreover umbelliprenin could inhibit McL[-1] protein. In conclusion umbelliprenin treatment modulates McL[-1] expression at both the transcriptional and posttranslational levels