ABSTRACT
Objective: The aim of the study was to evaluate the effect of extension of endocrown in pulp space and the effect of ferrule on the fracture resistance of anterior endocrowns made of nano-ceramic resin blocks. Material and Methods: Twenty-eight freshly extracted human upper central incisor teeth were prepared to receive CAD/CAM nanoceramic resin endocrowns with four different designs. The specimens were divided into two groups (n = 14) according to the extension of the endocrown inside root canal (A:short and B:long), then each group was further subdivided into another two subgroups (n = 7) according to the presence or absence of ferrule effect (1:ferrule and 2: no ferrule). Endocrowns were then fabricated using CEREC in lab and nanoceramic resin blocks (DENTSPLY Sirona, Germany) and LAVA ultimate (3M ESPE, Germany) blocks size 14L. Results: Two-way ANOVA showed that the ferrule (p > 0.0001) had statistically significant effect on the fracture resistance. However, the extension of the endocrown into the canal had no statistical significant effect on the fracture resistance (p = 0.837). The interactions between the independent variables (extension into the canal and ferrule) had statistically significant effect on the fracture resistance (p = 0.029). Load to fracture for group 1A was 439.53 N, 1B was 306.46 N, 2A was 516.29 N and 2B was 242.04 N. Conclusions: Fracture resistance was not improved by the long or short extensions of the endocrowns in the pulp space, however, the ferrule effect shows significant improvement of the fracture resistance of the nanoceramic resin endocrowns. (AU)
Objetivo: O objetivo deste estudo foi avaliar o efeito da extensão da coroa endocrown na câmara pulpar e o efeito férula na resistência à fratura de coroas endocrowns anteriores confeccionadas através de blocos de resina nanocerâmica. Material e Métodos: Vinte e oito dentes incisivos centrais superiores humanos recém-extraídos foram preparados para receber endocrowns de resina nanocerâmica CAD / CAM com quatro diferentes tipos de preparos. Os espécimes foram divididos em dois grupos (n = 14) de acordo com a extensão da endocrown dentro do canal radicular (A: curto e B: longo), então cada grupo foi subdividido em outros dois subgrupos (n = 7) de acordo com presença ou ausência do efeito férula (1: com férula e 2: sem férula). As endocrowns foram usinadas através do CEREC In Lab e blocos de resina nanocerâmica (DENTSPLY Sirona, Alemanha) e blocos de LAVA ultimate (3M ESPE, Alemanha) tamanho 14L. Resultados: ANOVA dois fatores demostrou que a férula (P> 0,0001) teve efeito estatisticamente significativo na resistência à fratura. No entanto, a extensão do endocrown no canal não teve efeito estatisticamente significativo na resistência à fratura (P = 0,837). As interações entre as variáveis independentes (extensão no canal e férula) tiveram efeito estatisticamente significativo na resistência à fratura (P = 0,029). A carga para fratura do grupo 1A foi de 439,53 N, 1B foi de 306,46 N, 2A foi de 516,29 N e 2B foi de 242,04 N. Conclusões: A resistência à fratura não foi melhorada pelas extensões longas ou curtas das endocrowns na câmara pulpar, no entanto, o efeito férula mostrou uma melhora significativa na resistência à fratura das coroas endocrowns de resina nanocerâmica (AU)
Subject(s)
Humans , Resin Cements , Dental Pulp Cavity , Flexural StrengthABSTRACT
<p><b>OBJECTIVE</b>To study the antioxidant and anti-inflammatory activity of Butia capitata (B. capitata) leaf extracts along with phytochemical analysis of the proposed bioactive constituents.</p><p><b>METHODS</b>Different successive extracts of B. capitata Becc. leaves were prepared with selective organic solvents and screened for their anti-inflammatory activities in tested animals and in-vitro antioxidant effect. An extensive phytochemical investigation of the bioactive extracts through paper chromatography, thin layer chromatography, column chromatography, gas-liquid chromatography (GLC), high pressure liquid chromatography and spectral analysis. GC-Mass, ultraviolet, hydrogen and carbon nuclear magnetic resonance, electron ionization-mass spectrometry, heteronuclear multiple bond correlation and heteronuclear multiple quantum correlation were carried out.</p><p><b>RESULTS</b>Results showed that different extracts possess promising antioxidant effect and significant anti-inflammatory activity with variable degrees. The results of the phytochemical investigation of the bioactive extracts revealed the presence of volatile substances, lipoidal matter, α-tocopherol, free sugars, polysaccharides and flavonoidal compounds.</p><p><b>CONCLUSIONS</b>B. capitata leaf extracts were shown to possess variable antioxidant effect, the most promising was methanol extract. Both polar and non polar extracts were proved to have anti-inflammatory activity, the non polar extract was superior in this respect. The bioactivity of the extracts was ascribed to the presence of flavonoids, sterols and α-tocopherol.</p>
ABSTRACT
Objective: To study the antioxidant and anti-inflammatory activity of Butia capitata (B. capitata) leaf extracts along with phytochemical analysis of the proposed bioactive constituents. Methods: Different successive extracts of B. capitata Becc. leaves were prepared with selective organic solvents and screened for their anti-inflammatory activities in tested animals and in-vitro antioxidant effect. An extensive phytochemical investigation of the bioactive extracts through paper chromatography, thin layer chromatography, column chromatography, gas-liquid chromatography (GLC), high pressure liquid chromatography and spectral analysis. GC-Mass, ultraviolet, hydrogen and carbon nuclear magnetic resonance, electron ionization-mass spectrometry, heteronuclear multiple bond correlation and heteronuclear multiple quantum correlation were carried out. Results: Results showed that different extracts possess promising antioxidant effect and significant anti-inflammatory activity with variable degrees. The results of the phytochemical investigation of the bioactive extracts revealed the presence of volatile substances, lipoidal matter, α-tocopherol, free sugars, polysaccharides and flavonoidal compounds. Conclusions: B. capitata leaf extracts were shown to possess variable antioxidant effect, the most promising was methanol extract. Both polar and non polar extracts were proved to have anti-inflammatory activity, the non polar extract was superior in this respect. The bioactivity of the extracts was ascribed to the presence of flavonoids, sterols and a-tocopherol.
ABSTRACT
Heparanase is an endoglycosidase that degrades heparin sulfate, the main polysaccharide constituent of the extracellular matrix and basement membrane. Expression of the heparanase gene is associated with the invasive, angiogenic, and metastatic potential of diverse malignant tumors and cell lines. to investigate possible relation/correlation between Heparanase gene expression and quantitation in pediatric Acute leukemia patients and clinicopathologic variables as well as patients outcome in an attempt to determine it's prognostic value and the possibility of using it as a new target for treatment. Forty pediatric acute leukemia patients [20 acute myeloid leukemia [AML] and 20 acute lymphoblastic leukemia [ALL] as well as 11 normal volunteers were analyzed for the expression and level of Heparanase gene using real time quantitative reverse transcriptase polymerase chain reaction [RTQ-PCR] to investigate a possible relation, association, or correlation with the clinical and laboratory features of patients at diagnosis, and patient outcome after treatment and follow up. Comparing the 3 groups as regards the Heparanase gene level there was high statistical significant difference [p<0.001] being maximum in AML and minimum in controls, with mean Relative quantitation [RQ] level 2336.2 +/- 10405.2 in AML ,median 8.0 and range [3.1-46543.0], while mean RQ in ALL was 1.7 +/- 1.0 ,median 1.7 and range [0.1-3.1] and in controls mean was 0.8+/-0.3, median 0.8 and range [0.4-1.4].Comparison between each 2 groups as regards heparanase level was of high statistically significant difference, p value being [p<0.001] when comparing AML/ALL and AML/controls and [p=0.035] when comparing ALL/controls. Cut off value for heparanase gene was calculated using Roc curve and was found to be 1.413 with 80% sensitivity and 100% specificity. According to this cut off level, 20/20 [100%] AML cases were heparanase positive, 12/20 [60%] [[ALL] cases were heparanase positive and 8/20 ALL patients were negative, while all controls [100%] were negative. This was of high statistical significance [p<0.001]. Comparing the overall survival [OS] of AML/ALL there was no statistically significant difference [p=0.2916], while comparing the disease free survival [DPS] of AML/ALL was of statistical significant difference [0.0312]. Comparing the final status of the disease [complete remission [CR]/ progressive disease [PD] or death] as regards the heparanase gene level RQ, showed a high statistical significant difference [p<0.005] with the level being higher in patients with PD/death. There was no significant correlation between all group and heparanase gene level as regards age, TLC, hemoglobin, platelets and peripheral blood blasts [p=0.353,0.704,0.844,0.54 and 0.097] respectively, while there was significant negative correlation on comparing bone marrow blast% and heparanase gene level [r=-0.408 and p=0.09]. Heparanse gene is expressed in acute leukemia being higher in AML than ALL and controls. Patients with higher heparanase gene showed poorer outcome. These findings suggest that heparanase gene may be a novel significant therapeutic target for acute leukemia
Subject(s)
Glucuronidase/genetics , Leukemia, Basophilic, Acute/genetics , Polymerase Chain Reaction/statistics & numerical dataABSTRACT
Radiation is a threat to health in work place and in general environment. Workers in radiation therapy department are exposed to variety type of occupational hazards. Health and safety measures compliance may decrease the health sequences of hazards to exposure. the research aimed to find out compliance to safety measures toward radiation hazards among the health team. the study were conducted at two radiation therapy settings affiliated to the largest cancer hospitals at Cairo governorate, National Cancer Institute and El demerdash hospital in Ain Shams university. A purposive sample [108] health team members in two settings were selected. [1] self administered questionnaire, [2] health team compliance observation checklist. finding revealed that, most of studied sample age were 35-45 years and had secondary education, most of them were had unsatisfactory knowledge and most of them were not compliance to health and safety measures compliance were more in safety practice. the study concluded there were lack of knowledge about radiation therapy, radiation hazards, and radiation safety measures specially technician, nurses, and clerk and low level of compliance to health and safety measures. the study recommended establish plans for periodical safety training courses for health team member in radiation setting to help in improving their practice and update their knowledge
Subject(s)
Humans , Male , Female , Chemical Safety/methods , Patient Care Team/statistics & numerical data , Safety/standards , Radiation Protection/methodsABSTRACT
Hepatitis C virus is a major health care problem in Egypt. One of its high risk groups are multi-transfused children suffering from chronic hemolytic anemia. In the current study we aimed to investigate the prevalence of hepatitis C virus status [HCV] in thalassemia major and sickle cell anemia patients who are regularly following at the pediatric hematology clinics, Cairo University. We also aimed to compare the current prevalence with previous studies conducted before the introduction of donor screening for HCV and so determine the effectiveness of screening currently in use. Two hundred and eighteen children were enrolled in the study. Most of them were thalassemia cases [n=168], while fifty were sickle cell disease patients. The study also included twenty healthy age- matched control to determine the base line risk in the general population. All patients and controls were investigated for the presence of HCV antibody using fourth generation ELISA techniques. The results showed that the over all HCV sero-prevalence rate was 40.5% in thalassemia patients and 50% in sickle cell disease patients, a much lower rate than previously reported before the era of donor screening. The risk for HCV infection in both thalassemia and sickle cell anemia increased with increased duration of transfusion. The mean transfusion duration in HCV positive thalassemia patients was [10.28 +/- 6 years] while among sero-negatives it was [3.27 +/- 4.32 years]. In sickle cell anemia group, the mean duration of transfusion was [7.04 +/- 4.29 y] among HCV positives versus [3.38 +/- 3 y] among HCV negatives and the relation was also highly significant. Serum ferritin was a significantly elevated in HCV positive cases both in sickle and thalassemia patients. The frequency of HCV infection has moderately declined since the introduction of blood screening techniques, however the current prevalence is still high and more efforts are needed to introduce new techniques that avoid the window gap and increase the sensitivity of used techniques. The duration of blood transfusion and high levels of serum ferritin were identified as possible risk factors for HCV infection in the studied groups
Subject(s)
Humans , Male , Female , Seroepidemiologic Studies , Anemia, Sickle Cell , beta-Thalassemia , Enzyme-Linked Immunosorbent AssayABSTRACT
Sickle cell disease [SCD] is associated with many cardiac abnormalities including increase cardiac output, pulmonary hypertension [PH] and myocardial perfusion abnormalities. Hydroxyurea [HU] is considered to be the most successful drug therapy for severe sickle cell disease. The objective is to study the echocardiographic cardiac abnormalities in patients with SCD on HU therapy comparing them with those on isolated blood transfusion regimen. Descriptive cross sectional case study was conducted on 29 cases of SCD on transfusion regimen [16 cases] and on HU therapy [13 cases]. Doppler echocardiographic assessment of left ventricular systolic and diastolic function, calculation of myocardial performance index [MPI] and left ventricular mass were done to all patients. Right ventricular MPI was higher in HU treated group with difference approaching significance [p = 0.06]. No significant correlation between HU dose and duration and left ventricular systolic or diastolic function, right ventricular diastolic function, or pulmonary artery pressure [PAP]. PH was present in 55% of SCD. No significant difference in PAP between HU and transfusion treated groups. Negative significant correlation between left ventricular deceleration time and estimated systolic pulmonary artery pressure [r=-0.37 p =0.04]. No deleterious echocardiographically detected cardiac effects were observed in HU treated SCD patients. HU had no effect on PH
Subject(s)
Humans , Male , Female , Hemoglobin SC Disease , Blood Transfusion , Hydroxyurea , Echocardiography , Ventricular Function, Left , Ventricular Function, Right , Hypertension, Pulmonary , Child , AdolescentABSTRACT
The aim of this study was to investigate the correlation between ABO blood groups, secretory status and chronic cholecystitis with or without peptic ulceration. This study was conducted on 30 patients [18 males and 12 females] proved by abdominal ultrasound to have chronic cholecystitis either calcular or non-calcular. Our results showed that there was a significant correlation between blood group A with positive secretory status and chronic cholecystitis either calcular or non-calcular. Also, chronic non-calcular cholecystitis was frequent in patients with blood group 0 with negative secretory status. Duodenal ulcer was significantly present in patients with blood group A with positive secretory status and blood group 0 with negative secretory status when compared with other blood groups. From the above results we suggest that both chronic cholecystitis and duodenal ulcer might have the same genetic predisposition