ABSTRACT
Os autores apresentam um caso de transplante hepático em dominó realizado com nova técnica cirúrgica. A paciente, portadora de Polineuropatia Amiloidótica Familiar Tipo I (PAF) foi submetida a hepatectomia pela técnica piggback, recebendo fígado de cadáver. O segundo paciente com cirrose vírus B e carcinoma hepatocelular, receptor dominó, também foi submetido a hepatectomia com preservação da veia cava recebendo enxerto após reconstruções vasculares (venosa e arterial). Ambos tiveram alta sem intercorrências. Os relatos demonstram que a modalidade de transplante hepático dominó é factível com a técnica de duplo piggback, prescindindo do desvio porto-cava-axilar
Subject(s)
Female , Adult , Digestive System Surgical Procedures , Liver Transplantation/methods , Specialties, SurgicalABSTRACT
The 18-kDa protein from Mycobacterium leprae is a major target for the immune response in leprosy. We have developed a system to express this antigen in yeast as a fusion protein with the C-terminal region of the yeast membrane protein GAS1, which would render the recombinant protein anchored to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Cells lacking the GAS1 gene and transformed with the hybrid 18-kDa-GAS1 construct express a polypeptide that reacts with an 18-kDa-specific monoclonal antibody. In addition, these cells react with an alpha-CRD antibody after GPI-PLC treatment. The non-transformed cells are negative. These data indicate that our system may be suitable for the expression of foreign proteins in yeast in a GPI-anchored form